Mixtures comprising a Bacillus strain and a pesticide

ABSTRACT

The present invention relates to pesticidal mixtures comprising as active components the  Bacillus  strains AP-136, AP-188, APP-218, AP-219, AP-295, AP-209 and/or AP-217 as defined herein and a pesticide II as defined herein and respective agricultural uses thereof.

This application is a National Stage application of InternationalApplication No. PCT/EP2015/060590, filed May 13, 2015, which claims thebenefit of U.S. Provisional Application No. 62/002,210, filed May 23,2014, and U.S. Provisional Application No. 62/002,949, filed May 26,2014. This application also claims priority under 35 U.S.C. § 119 toEuropean Patent Application No. 14175139.6, filed Jul. 1, 2014.

The present invention relates to mixtures comprising, as activecomponents the Bacillus strains AP-136, AP-188, APP-218, AP-219, AP-295,AP-209 and/or AP-217 and a pesticide.

This microorganisms Bacillus amyloliquefaciens AP-136, AP-188, AP-218,AP-219, AP-295, Bacillus mojavensis AP-209 and Bacillus solisalsi AP-217are known from US 2012/0149571, the content of which is incorporated byreference in its entirety. They have been described therein specificallyas inoculants for promoting the plant defense response againstherbivorous insects.

The strain B. amyloliquefaciens AP-136 has been deposited with theUnited States Department of Agriculture (USDA) on Nov. 5, 2009 underaccession number (acc. no.) NRRL B-50330 and on Dec. 2, 2011 under acc.no. NRRL B-50614. The strain B. amyloliquefaciens AP-188 has beendeposited with USDA on Nov. 5, 2009 under acc. no. NRRL B-50331 and onDec. 2, 2011 under acc. no. NRRL B-50615. The strain B.amyloliquefaciens AP-218 has been deposited with USDA on Dec. 2, 2011under acc. no. NRRL B-50618. The strain B. amyloliquefaciens AP-219 hasbeen deposited with USDA on Nov. 5, 2009 under acc. no. NRRL B-50332 andon Dec. 2, 2011 under acc. no. NRRL B-50619. The strain B.amyloliquefaciens AP-295 has been deposited with USDA on Nov. 5, 2009under acc. no. NRRL B-50333 and on Dec. 2, 2011 under acc. no. NRRLB-50620. The strain B. mojavensis AP-209 has been deposited with USDA onDec. 2, 2011 under acc. no. NRRL B-50616. The strain B. solisalsi AP-217has been deposited with USDA on Dec. 2, 2011 under acc. no. NRRLB-50617.

A pesticide is generally a chemical or biological agent (such as avirus, bacterium, antimicrobial or disinfectant) that through its effectdeters, incapacitates, kills or otherwise discourages pests. Targetpests can include insects, plant pathogens, weeds, mollusks, birds,mammals, fish, nematodes (roundworms), and microbes that destroyproperty, cause nuisance, spread disease or are vectors for disease.

Biopesticides have been defined as a form of pesticides based onmicro-organisms (bacteria, fungi, viruses, nematodes, etc.) or naturalproducts (compounds, such as metabolites, proteins, or extracts frombiological or other natural sources) (U.S. Environmental ProtectionAgency: http://www.epa.gov/pesticides/biopesticides/). Biopesticidesfall into two major classes, microbial and biochemical pesticides:

(1) Microbial pesticides consist of bacteria, fungi or viruses (andoften include the metabolites that bacteria and fungi produce).

(2) Biochemical pesticides are naturally occurring substances or orstructurally-similar and functionally identical to a naturally-occurringsubstance and extracts from biological sources that control pests orprovide other crop protection uses as defined below, but have non-toxicmode of actions and are relatively non-toxic to mammals.

Biopesticides for use against crop diseases have already establishedthemselves on a variety of crops. For example, biopesticides alreadyplay an important role in controlling downy mildew diseases. Theirbenefits include: a 0-Day Pre-Harvest Interval, the ability to use undermoderate to severe disease pressure, and the ability to use in mixtureor in a rotational program with other registered pesticides.

However, biopesticides under certain conditions can also havedisadvantages such as high specificity: which may require an exactidentification of the pest/pathogen and the use of multiple products tobe used, slow speed of action (thus making them unsuitable if a pestoutbreak is an immediate threat to a crop), variable efficacy due to theinfluences of various biotic and abiotic factors (since biopesticidesare usually living organisms, which bring about pest/pathogen control bymultiplying within the target insect pest/pathogen) and resistancedevelopment.

Practical agricultural experience has shown that the repeated andexclusive application of an individual active component in the controlof harmful fungi, insects or other pests leads in many cases to a rapidselection of those fungus strains or pest isolates which have developednatural or adapted resistance against the active component in question.Effective control of these fungi, insects or other pests with the activecomponent in question is then no longer possible. Another typicalproblem arising in the field of pest control lies in the need to reducethe dosage rates of the active ingredient in order to reduce or avoidunfavorable environmental or toxicological effects whilst still allowingeffective pest control.

It is an object of the present invention overcome the abovementioneddisadvantages and to provide, with a view to effective resistancemanagement and effective control of phytopathogenic harmful fungi,insects or other pests or to effective plant growth regulation, atapplication rates which are as low as possible, compositions which, at areduced total amount of active compounds applied, have improved activityagainst the harmful fungi, insects or other pests or improved plantgrowth regulating activity (synergistic mixtures) and a broadenedactivity spectrum, in particular for certain indications.

This is particularly visible if application rates for thebeforementioned mixtures of pesticides are used where the individualcomponents show no or virtually no activity. The invention can alsoresult in an advantageous behavior during formulation or during use, forexample during grinding, sieving, emulsifying, dissolving or dispensing;improved storage stability and light stability, advantageous residueformation, improved toxicological or ecotoxicological behaviour,improved properties of the plant, for example better growth, increasedharvest yields, a better developed root system, a larger leaf area,greener leaves, stronger shoots, less seed required, lowerphytotoxicity, mobilization of the defense system of the plant, improvedcompatibility with plants.

It was therefore also an object of the present invention to providepesticidal mixtures which solve the problems of reducing the dosage rateand/or enhancing the spectrum of activity and/or combining knock-downactivity with prolonged control and/or to resistance management and/orpromoting (increasing) the health of plants.

We have accordingly found that this object is achieved by the mixturesand compositions defined herein, comprising at least one microorganismof the genus Bacillus seleted from B. amyloliquefaciens AP-136 (NRRLB-50330; NRRL B-50614), B. amyloliquefaciens AP-188 (NRRL B-50331; NRRLB-50615), B. amyloliquefaciens AP-218 (NRRL B-50618), B.amyloliquefaciens AP-219 (NRRL B-50332, NRRL B-50619), B.amyloliquefaciens AP-295 (NRRL B-50333, NRRL B-50620) B. mojavensisAP-209 (NRRL B-50616), and B. solisalsi AP-217 (NRRL B-50617).

Thus, the present invention relates to mixtures comprising, as activecomponents

-   1) at least one microorganism I of the genus Bacillus selected from:

B. amyloliquefaciens AP-136 (NRRL B-50330; NRRL B-50614),

B. amyloliquefaciens AP-188 (NRRL B-50331; NRRL B-50615),

B. amyloliquefaciens AP-218 (NRRL B-50618),

B. amyloliquefaciens AP-219 (NRRL B-50332, NRRL B-50619),

B. amyloliquefaciens AP-295 (NRRL B-50333, NRRL B-50620)

B. mojavensis AP-209 (NRRL B-50616), and

B. solisalsi AP-217 (NRRL B-50617);

and

-   2) at least one pesticide II from the groups A) to O) selected from:-   A) Respiration inhibitors    -   Inhibitors of complex III at Q_(o) site selected from:        azoxystrobin (A.1.1), coumethoxystrobin (A.1.2), coumoxystrobin        (A.1.3), dimoxystrobin (A.1.4), enestroburin (A.1.5),        fenaminstrobin (A.1.6), fenoxystrobin/flufenoxystrobin (A.1.7),        fluoxastrobin (A.1.8), kresoxim-methyl (A.1.9), mandestrobin        (A.1.10), metominostrobin (A.1.11), orysastrobin (A.1.12),        picoxystrobin (A.1.13), pyraclostrobin (A.1.14), pyrametostrobin        (A.1.15), pyraoxystrobin (A.1.16), trifloxystrobin (A.1.17),        2-(2-(3-(2,6-dichlorophenyl)-1-methyl-allylideneaminooxymethyl)-phenyl)-2-methoxyimino-N-methyl-acetamide        (A.1.18), pyribencarb (A.1.19), triclopyricarb/chlorodincarb        (A.1.20), famoxadone (A.1.21), fenamidone (A.1.21),        methyl-N-[2-[(1,4-dimethyl-5-phenyl-pyrazol-3-yl)oxylmethyl]phenyl]N-methoxy-carbamate        (A.1.22),        1-[3-chloro-2-[[[1-(4-chlorophenyl)-1H-pyrazol-3-yl]oxy]methyl]phenyl]-1,4-dihydro-4-methyl-5H-tetrazol-5-one        (A.1.23),        (2E,3Z)-5-[[1-(2,4-dichlorophenyl)-1H-pyrazol-3-yl]oxy]-2-(methoxyimino)-N,3-dimethyl-pent-3-enamide        (A.1.24).        (2E,3Z)-5-[[1-(4-chlorophenyl)-1H-pyrazol-3-yl]oxy]-2-(methoxyimino)-N,3-dimethyl-pent-3-enamide        (A.1.25);    -   inhibitors of complex III at Q site selected from: cyazofamid        (A.2.1), amisulbrom (A.2.2),        [(3S,6S,7R,8R)-8-benzyl-3-[(3-acetoxy-4-methoxy-pyridine-2-carbonyl)amino]-6-methyl-4,9-dioxo-1,5-dioxonan-7-yl]2-methylpropanoate        (A.2.3),        [(3S,6S,7R,8R)-8-benzyl-3-[[3-(acet-oxymethoxy)-4-methoxy-pyridine-2-carbonyl]amino]-6-methyl-4,9-dioxo-1,5-dioxonan-7-yl]        2-methylpropanoate (A.2.4),        [(3S,6S,7R,8R)-8-benzyl-3-[(3-isobutoxycarbonyloxy-4-meth-oxy-pyridine-2-carbonyl)amino]-6-methyl-4,9-dioxo-1,5-dioxonan-7-yl]        2-methylpropanoate (A.2.5),        [(3S,6S,7R,8R)-8-benzyl-3-[[3-(1,3-benzodioxol-5-ylmethoxy)-4-methoxy-pyridine-2-carbonyl]amino]-6-methyl-4,9-dioxo-1,5-dioxonan-7-yl]        2-methylpropanoate (A.2.6);        (3S,6S,7R,8R)-3-[[(3-hydroxy-4-methoxy-2-pyridinyl)carbonyl]amino]-6-methyl-4,9-dioxo-8-(phenylmethyl)-1,5-dioxonan-7-yl        2-methylpropanoate (A.2.7);    -   inhibitors of complex II selected from: benodanil (A.3.1),        benzovindiflupyr (A.3.2), bixafen (A.3.3), boscalid (A.3.4),        carboxin (A.3.5), fenfuram (A.3.6), fluopyram (A.3.7),        flutolanil (A.3.8), fluxapyroxad (A.3.9), furametpyr (A.3.10),        isofetamid (A.3.11), isopyrazam (A.3.12), mepronil (A.3.13),        oxycarboxin (A.3.14), penflufen (A.3.14), penthiopyrad (A.3.15),        sedaxane (A.3.16), tecloftalam (A.3.17), thifluzamide (A.3.18),        N-(4′-trifluoromethylthiobiphenyl-2-yl)-3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide        (A.3.19),        N-(2-(1,3,3-trimethyl-butyl)-phenyl)-1,3-dimethyl-5-fluoro-1H-pyrazole-4-carboxamide        (A.3.20),        3-(difluoromethyl)-1-methyl-N-(1,1,3-trimethylindan-4-yl)pyrazole-4-carboxamide        (A.3.21),        3-(trifluoromethyl)-1-methyl-N-(1,1,3-trimethylindan-4-yl)pyrazole-4-carboxamide        (A.3.22),        1,3-dimethyl-N-(1,1,3-trimethylindan-4-yl)pyrazole-4-carboxamide        (A.3.23),        3-(trifluoromethyl)-1,5-dimethyl-N-(1,1,3-trimethylindan-4-yl)pyrazole-4-carboxamide        (A.3.24),        1,3,5-trimethyl-N-(1,1,3-trimethylindan-4-yl)pyrazole-4-carboxamide        (A.3.25),        N-(7-fluoro-1,1,3-trimethyl-indan-4-yl)-1,3-dimethyl-pyrazole-4-carboxamide        (A.3.26),        N-[2-(2,4-dichlorophenyl)-2-methoxy-1-methyl-ethyl]-3-(difluoromethyl)-1-methyl-pyrazole-4-carboxamide        (A.3.27);    -   other respiration inhibitors selected from: diflumetorim        (A.4.1),        (5,8-difluoroquinazolin-4-yl)-{2-[2-fluoro-4-(4-trifluoromethylpyridin-2-yloxy)-phenyl]-ethyl}-amine(A.4.2);        nitrophenyl derivates: binapacryl (A.4.3), dinobuton (A.4.4),        dinocap (A.4.5), fluazinam (A.4.6); ferimzone (A.4.7);        organometal compounds: fentin salts, such as fentin-acetate        (A.4.8), fentin chloride (A.4.9) or fentin hydroxide (A.4.10);        ametoctradin (A.4.11); and silthiofam (A.4.12);-   B) Sterol biosynthesis inhibitors (SBI fungicides)    -   C14 demethylase inhibitors (DMI fungicides) selected from:        triazoles: azaconazole (B.1.1), bitertanol (B.1.2),        bromuconazole (B.1.3), cyproconazole (B.1.4), difenoconazole        (B.1.5), diniconazole (B.1.6), diniconazole-M (B.1.7),        epoxiconazole (B.1.8), fenbuconazole (B.1.9), fluquinconazole        (B.1.10), flusilazole (B.1.11), flutriafol (B.1.12),        hexaconazole (B.1.13), imibenconazole (B.1.14), ipconazole        (B.1.15), metconazole (B.1.17), myclobutanil (B.1.18),        oxpoconazole (B.1.19), paclobutrazole (B.1.20), penconazole        (B.1.21), propiconazole (B.1.22), prothioconazole (B.1.23),        simeconazole (B.1.24), tebuconazole (B.1.25), tetraconazole        (B.1.26), triadimefon (B.1.27), triadimenol (B.1.28),        triticonazole (B.1.29), uniconazole (B.1.30),        1-[rel-(2S;3R)-3-(2-chlorophenyl)-2-(2,4-difluorophenyl)-oxiranyl-methyl]-5-thiocyanato-1H-[1,2,4]triazolo        (B.1.31),        2-[rel-(2S;3R)-3-(2-chlorophenyl)-2-(2,4-difluorophenyl)-oxiranylmethyl]-2H-[1,2,4]triazole-3-thiol        (B.1.32),        2-[2-chloro-4-(4-chloro-phenoxy)phenyl]-1-(1,2,4-triazol-1-yl)pentan-2-ol        (B.1.33),        1-[4-(4-chlorophenoxy)-2-(tri-fluoromethyl)phenyl]-1-cyclopropyl-2-(1,2,4-triazol-1-ypethanol        (B.1.34),        2-[4-(4-chloro-phenoxy)-2-(trifluoromethyl)phenyl]-1-(1,2,4-triazol-1-yl)butan-2-ol        (B.1.35),        2-[2-chloro-4-(4-chlorophenoxy)phenyl]-1-(1,2,4-triazol-1-yl)butan-2-ol        (B.1.36),        2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-3-methyl-1-(1,2,4-triazol-1-yl)butan-2-ol        (B.1.37),        2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1-(1,2,4-triazol-1-yl)propan-2-ol        (B.1.38),        2-[2-chloro-4-(4-chlorophenoxy)phenyl]-3-methyl-1-(1,2,4-triazol-1-yl)butan-2-ol        (B.1.39),        2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1-(1,2,4-triazol-1-yl)pentan-2-ol        (B.1.40),        2-[4-(4-fluorophenoxy)-2-(trifluoromethyl)phenyl]-1-(1,2,4-triazol-1-yl)propan-2-ol        (B.1.41)),        2-[2-chloro-4-(4-chlorophenoxy)phenyl]-1-(1,2,4-triazol-1-yl)pent-3-yn-2-ol        (B.1.51); imidazoles: imazalil (B.1.42), pefurazoate (B.1.43),        prochloraz (B.1.44), triflumizol (B.1.45); pyrimidines,        pyridines and piperazines: fenarimol (B.1.46), nuarimol        (B.1.47), pyrifenox (B.1.48), triforine (B.1.49),        [3-(4-chloro-2-fluoro-phenyl)-5-(2,4-difluorophenypisoxazol-4-yl]-(3-pyridyl)methanol        (B.1.50);    -   Delta14-reductase inhibitors selected from: aldimorph (B.2.1),        dodemorph (B.2.2), dodemorph-acetate (B.2.3), fenpropimorph        (B.2.4), tridemorph (B.2.5), fenpropidin (B.2.6), piperalin        (B.2.7), spiroxamine (B.2.8);    -   Inhibitor of 3-keto reductase: fenhexamid (B.3.1);-   C) Nucleic acid synthesis inhibitors    -   phenylamides or acyl amino acid fungicides selected from:        benalaxyl (C.1.1), benalaxyl-M (C.1.2), kiralaxyl (C.1.3),        metalaxyl (C.1.4), metalaxyl-M (mefenoxam, C.1.5), ofurace        (C.1.6), oxadixyl (C.1.7);    -   others selected from: hymexazole (C.2.1), octhilinone (C.2.2),        oxolinic acid (C.2.3), bupirimate (C.2.4), 5-fluorocytosine        (C.2.5), 5-fluoro-2-(p-tolylmethoxy)pyrimidin-4-amine (C.2.6),        5-fluoro-2-(4-fluorophenylmethoxy)pyrimidin-4-amine (C.2.7);-   D) Inhibitors of cell division and cytoskeleton    -   tubulin inhibitors selected from: benomyl (D1.1), carbendazim        (D1.2), fuberidazole (D1.3), thiabendazole (D1.4),        thiophanate-methyl (D1.5); triazolopyrimidines:        5-chloro-7-(4-methyl-piperidin-1-yl)-6-(2,4,6-trifluorophenyl)-[1,2,4]triazolo[1,5-a]pyrimidine        (D1.6);    -   other cell division inhibitors selected from: diethofencarb        (D2.1), ethaboxam (D2.2), pencycuron (D2.3), fluopicolide        (D2.4), zoxamide (D2.5), metrafenone (D2.6), pyriofenone (D2.7);-   E) Inhibitors of amino acid and protein synthesis    -   methionine synthesis inhibitors (anilino-pyrimidines) selected        from: cyprodinil (E.1.1), mepanipyrim (E.1.2), pyrimethanil        (E.1.3);    -   protein synthesis inhibitors selected from: blasticidin-S        (E.2.1), kasugamycin (E.2.2), kasugamycin hydrochloride-hydrate        (E.2.3), mildiomycin (E.2.4), streptomycin (E.2.5),        oxytetracyclin (E.2.6), polyoxine (E.2.7), validamycin A        (E.2.8);-   F) Signal transduction inhibitors    -   MAP/histidine kinase inhibitors selected from: fluoroimid        (F.1.1), iprodione (F.1.2), procymidone (F.1.3), vinclozolin        (F.1.4), fenpiclonil (F.1.5), fludioxonil (F.1.6);    -   G protein inhibitor: quinoxyfen (F.2.1);-   G) Lipid and membrane synthesis inhibitors    -   Phospholipid biosynthesis inhibitors selected from: edifenphos        (G.1.1), iprobenfos (G.1.2), pyrazophos (G.1.3), isoprothiolane        (G.1.4);    -   lipid peroxidation compounds selected from: dicloran (G.2.1),        quintozene (G.2.2), tecnazene (G.2.3), tolclofos-methyl (G.2.4),        biphenyl (G.2.5), chloroneb (G.2.6), etridiazole (G.2.7);    -   phospholipid biosynthesis and cell wall deposition: dimethomorph        (G.3.1), flumorph (G.3.2), mandipropamid (G.3.3), pyrimorph        (G.3.4), benthiavalicarb (G.3.5), iprovalicarb (G.3.6),        valifenalate (G.3.7) and        N-(1-(1-(4-cyano-phenyl)ethanesulfonyl)-but-2-yl) carbamic        acid-(4-fluorophenyl) ester (G.3.8);    -   compound affecting cell membrane permeability and fatty acides:        propamocarb (G.4.1);    -   fatty acid amide hydrolase inhibitors selected from:        oxathiapiprolin (G.5.1),        2-{3-[2-(1-{[3,5-bis(difluoromethyl-1H-pyrazol-1-yl]acetyl}piperidin-4-yl)-1,3-thiazol-4-yl]-4,5-dihydro-1,2-oxazol-5-yl}phenyl        methanesulfonate (G.5.2),        2-{3-[2-(1-{[3,5-bis(difluoromethyl)-1H-pyrazol-1-yl]acetyl}piperidin-4-yl)        1,3-thiazol-4-yl]-4,5-dihydro-1,2-oxazol-5-yl}-3-chlorophenyl        methanesulfonate (G.5.3);-   H) Inhibitors with multi site action    -   inorganic active substances selected from: Bordeaux mixture        (H.1.1), copper acetate (H.1.2), copper hydroxide (H.1.3),        copper oxychloride (H.1.4), basic copper sulfate (H.1.5), sulfur        (H.1.6);    -   thio- and dithiocarbamates selected from: ferbam (H.2.1),        mancozeb (H.2.2), maneb (H.2.3), metam (H.2.4), metiram (H.2.5),        propineb (H.2.6), thiram (H.2.7), zineb (H.2.8), ziram (H.2.9);    -   organochlorine compounds selected from: anilazine (H.3.1),        chlorothalonil (H.3.2), captafol (H.3.3), captan (H.3.4), folpet        (H.3.5), dichlofluanid (H.3.6), dichlorophen (H.3.7),        hexachlorobenzene (H.3.8), pentachlorphenole (H.3.9) and its        salts, phthalide (H.3.10), tolylfluanid (H.3.11),        N-(4-chloro-2-nitro-phenyl)-N-ethyl-4-methyl-benzenesulfonamide        (H.3.12);    -   guanidines and others selected from: guanidine (H.4.1), dodine        (H.4.2), dodine free base (H.4.3), guazatine (H.4.4),        guazatine-acetate (H.4.5), iminoctadine (H.4.6),        iminoctadine-triacetate (H.4.7), iminoctadine-tris(albesilate)        (H.4.8), dithianon (H.4.9),        2,6-dimethyl-1H,5H-[1,4]dithiino[2,3-c:5,6-c′]dipyrrole-1,3,5,7(2H,6H)-tetraone        (H.4.10);-   I) Cell wall synthesis inhibitors    -   inhibitors of glucan synthesis selected from: validamycin        (I.1.1), polyoxin B (I.1.2);    -   melanin synthesis inhibitors: pyroquilon (I.2.1), tricyclazole        (I.2.2), carpropamid (I.2.3), dicyclomet (I.2.4), fenoxanil        (I.2.5);-   J) Plant defence inducers selected from: acibenzolar-S-methyl    (J.1.1), probenazole (J.1.2), isotianil (J.1.3), tiadinil (J.1.4),    prohexadione-calcium (J.1.5); phosphonates: fosetyl (J.1.6),    fosetyl-aluminum (J.1.7), phosphorous acid and its salts (J.1.8),    potassium or sodium bicarbonate (J.1.9);-   K) Unknown mode of action selected from: bronopol (K.1.1),    chinomethionat (K.1.2), cyflufenamid (K.1.3), cymoxanil (K.1.4),    dazomet (K.1.5), debacarb (K.1.6), diclomezine (K.1.7), difenzoquat    (K.1.8), difenzoquat-methylsulfate (K.1.9), diphenylamin (K.1.10),    fenpyrazamine (K.1.11), flumetover (K.1.12), flusulfamide (K.1.13),    flutianil (K.1.14), methasulfocarb (K.1.15), nitrapyrin (K.1.16),    nitrothal-isopropyl (K.1.18), oxathiapiprolin (K.1.19), tolprocarb    (K.1.20), oxin-copper (K.1.21), proquinazid (K.1.22), tebufloquin    (K.1.23), tecloftalam (K.1.24), triazoxide (K.1.25),    2-butoxy-6-iodo-3-propylchromen-4-one (K.1.26),    2-[3,5-bis(difluoromethyl)-1H-pyrazol-1-yl]-1-[4-(4-{5-[2-(prop-2-yn-1-yloxy)phenyl]-4,5-dihydro-1,2-oxazol-3-yl}-1,3-thiazol-2-yl)piperidin-1-yl]ethanone    (K.1.27),    2-[3,5-bis(di-fluoromethyl)-1H-pyrazol-1-yl]-1-[4-(4-{5-[2-fluoro-6-(prop-2-yn-1-yloxy)phenyl]-4,5-dihydro-1,2-oxazol-3-yl}-1,3-thiazol-2-yl)piperidin-1-yl]ethanone    (K.1.28),    2-[3,5-bis(difluoromethyl)-1H-pyrazol-1-yl]-1-[4-(4-{5-[2-chloro-6-(prop-2-yn-1-yloxy)phenyl]-4,5-dihydro-1,2-oxazol-3-yl}-1,3-thiazol-2-yl)piperidin-1-yl]ethanone    (K.1.29),    N-(cyclopropylmethoxyimino-(6-difluoro-methoxy-2,3-difluoro-phenyl)-methyl)-2-phenyl    acetamide (K.1.30),    N′-(4-(4-chloro-3-trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N-methyl    formamidine (K.1.31),    N′-(4-(4-fluoro-3-trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N-methyl    formamidine (K.1.32),    N′-(2-methyl-5-trifluoromethyl-4-(3-trimethylsilanyl-propoxy)-phenyl)-N-ethyl-N-methyl    formamidine (K.1.33),    N′-(5-difluoromethyl-2-methyl-4-(3-trimethylsilanyl-propoxy)-phenyl)-N-ethyl-N-methyl    formamidine (K.1.34), methoxy-acetic acid    6-tert-butyl-8-fluoro-2,3-dimethyl-quinolin-4-yl ester (K.1.35),    3-[5-(4-methylphenyl)-2,3-dimethyl-isoxazolidin-3-yl]-pyridine    (K.1.36),    3-[5-(4-chloro-phenyl)-2,3-dimethyl-isoxazolidin-3-yl]pyridine    (pyrisoxazole) (K.1.37), N-(6-methoxy-pyridin-3-yl)    cyclopropanecarboxylic acid amide (K.1.38),    5-chloro-1-(4,6-dimethoxy-pyrimidin-2-yl)-2-methyl-1H-benzoimidazole    (K.1.39),    2-(4-chloro-phenyl)-N-[4-(3,4-dimethoxy-phenyl)-isoxazol-5-yl]-2-prop-2-ynyloxy-acetamide,    ethyl (Z)-3-amino-2-cyano-3-phenyl-prop-2-enoate (K.1.40),    picarbutrazox (K.1.41), pentyl    N-[6-[[(Z)-[(1-methyltetrazol-5-yl)-phenyl-methylene]amino]oxymethyl]-2-pyridyl]carbamate    (K.1.42),    2-[2-[(7,8-difluoro-2-methyl-3-quinolyl)oxy]-6-fluoro-phenyl]propan-2-ol    (K.1.43),    2-[2-fluoro-6-[(8-fluoro-2-methyl-3-quinolyl)oxy]phen-yl]propan-2-ol    (K.1.44),    3-(5-fluoro-3,3,4,4-tetramethyl-3,4-dihydroisoquinolin-1-yl)quinoline    (K.1.45),    3-(4,4-difluoro-3,3-dimethyl-3,4-dihydroisoquinolin-1-yl)quinoline    (K.1.46),    3-(4,4,5-trifluoro-3,3-dimethyl-3,4-dihydroisoquinolin-1-yl)quinoline    (K.1.47), 9-fluoro-2,2-dimethyl-5-(3-quinolyl)-3H-1,4-benzoxazepine    (K.1.48);-   L) Biopesticides    -   L1) Microbial pesticides with fungicidal, bactericidal,        viricidal and/or plant defense activator activity selected from:        Ampelomyces quisqualis, Aspergillus flavus, Aureobasidium        pullulans, Bacillus altitudinis, B. amyloliquefaciens, B.        megaterium, B. mojavensis, B. mycoides, B. pumilus, B.        simplex, B. solisalsi; B. subtilis, B. subtilis var.        amyloliquefaciens, Candida oleophlla, C. saitoana, Clavibacter        michiganensis (bacteriophages), Coniothyrium minitans,        Cryphonectria parasitica, Cryptococcus albidus, Dilophosphora        alopecuri, Fusarium oxysporum, Clonostachys rosea f. catenulate        (also named Gliocladium catenulatum), Gliocladium roseum,        Lysobacter antibioticus, L. enzymogenes, Metschnikowia        fructicola, Microdochium dimerum, Microsphaeropsis ochracea,        Muscodor albus, Paenibacillus alvei, Paenibacillus polymyxa, P.        agglomerans, Pantoea vagans, Penicillium bilaiae, Phlebiopsis        gigantea, Pseudomonas sp., Pseudomonas chloraphis, P.        fluorescens, P. putida, Pseudozyma flucculosa, Pichia anomala,        Pythium oligandrum, Sphaerodes mycoparasitica, Streptomyces        griseoviridis, S. lydicus, S. violaceusniger, Talaromyces        flavus, Typhula phacorrhiza, Ulocladium oudemansii, Verticillium        dahlia, zucchini yellow mosaic virus (avirulent strain);    -   L2) Biochemical pesticides with fungicidal, bactericidal,        viricidal and/or plant defense activator activity selected from:        chitosan (hydrolysate), harpin protein, laminarin, Menhaden fish        oil, natamycin, Plum pox virus coat protein, potassium or sodium        bicarbonate, Reynoutria sachalinensis extract, salicylic acid,        tea tree oil;    -   L3) Microbial pesticides with insecticidal, acaricidal,        molluscidal and/or nematicidal activity selected from:        Agrobacterium radiobacter, Bacillus cereus, B. firmus, B.        thuringiensis, B. thuringiensis ssp. aizawai, B. t. ssp.        israelensis, B. t. ssp. galleriae, B. t. ssp. kurstaki, B. t.        ssp. tenebrionis, Beauveria bassiana, B. brongniartii,        Burkholderia spp., Chromobacterium subtsugae, Cydia pomonella        granulovirus (CpGV), Cryptophlebia leucotreta granulovirus        (CrleGV), Flavobacterium spp., Helicoverpa armigera        nucleopolyhedrovirus (HearNPV), Heterorhabditis bacteriophora,        Isaria fumosorosea, Lecanicillium longisporum, L. muscarium,        Metarhizium anisopliaze, Metarhizium anisopliae var.        anisopliae, M. anisopliae var. acridum, Nomuraea rileyi;        Paecilomyces lilacinus, Paenibacillus popilliae, Pasteuria        spp., P. nishizawae, P. penetrans, P. ramosa, P. thornea, P.        usgae, Pseudomonas fluorescens, Spodoptera littoralis        nucleopolyhedrovirus (SpliNPV), Steinernema carpocapsae, S.        feltiae, S. kraussei, Streptomyces galbus, S. microflavus;    -   L4) Biochemical pesticides with insecticidal, acaricidal,        molluscidal, pheromone and/or nematicidal activity selected        from: L-carvone, citral, (E,Z)-7,9-dodecadien-1-yl acetate,        ethyl formate, (E,Z)-2,4-ethyl decadienoate (pear ester),        (Z,Z,E)-7,11,13-hexadecatrienal, heptyl butyrate, isopropyl        myristate, lavanulylsenecioate, cis-jasmone, 2-methyl 1-butanol,        methyl eugenol, methyl jasmonate, (E,Z)-2,13-octadecadien-1-ol,        (E,Z)-2,13-octadecadien-1-ol acetate,        (E,Z)-3,13-octadecadien-1-ol, R-1-octen-3-ol, pentatermanone,        potassium silicate, sorbitol actanoate,        (E,Z,Z)-3,8,11-tetradecatrienyl acetate,        (Z,E)-9,12-tetradecadien-1-ylacetate, Z-7-tetradecen-2-one,        Z-9-tetradecen-1-ylacetate, Z-11-tetradecenal,        Z-11-tetradecen-1-ol, Acacia negra extract, extract of        grapefruit seeds and pulp, extract of Chenopodium ambrosiodes,        Catnip oil, Neem oil, Quillay extract, Tagetes oil;    -   L5) Microbial pesticides with plant stress reducing, plant        growth regulator, plant growth promoting and/or yield enhancing        activity selected from: Azospirillum amazonense, A.        brasilense, A. lipoferum, A. irakense, A. halopraeferens,        Bradyrhizobium spp., B. elkanii, B. japonicum, B.        liaoningense, B. lupini, Delftia acidovorans, Glomus        intraradices, Mesorhizobium spp., Rhizobium leguminosarum bv.        phaseoli, R. I. bv. trifolii, R. I. bv. viciae, R. tropici,        Sinorhizobium meliloti,    -   L6) Biochemical pesticides with plant stress reducing, plant        growth regulator and/or plant yield enhancing activity selected        from: abscisic acid, aluminium silicate (kaolin), 3-decen-2-one,        formononetin, genistein, hesperetin, homobrassinolide, humates,        jasmonic acid and its salts or derivatives thereof,        lysophosphatidyl ethanolamine, naringenin, polymeric polyhydroxy        acid, Ascophyllum nodosum (Norwegian kelp, Brown kelp) extract        and Ecklonia maxima (kelp) extract;-   M) Growth regulators selected from: abscisic acid (M.1.1),    amidochlor, ancymidol, 6-benzyl-aminopurine, brassinolide, butralin,    chlormequat, chlormequat chloride, choline chloride, cyclanilide,    daminozide, dikegulac, dimethipin, 2,6-dimethylpuridine, ethephon,    flumetralin, flurprimidol, fluthiacet, forchlorfenuron, gibberellic    acid, inabenfide, indole-3-acetic acid, maleic hydrazide,    mefluidide, mepiquat, mepiquat chloride, naphthaleneacetic acid,    N-6-benzyladenine, paclobutrazol, prohexadione,    prohexadione-calcium, prohydrojasmon, thidiazuron, triapenthenol,    tributyl phosphorotrithioate, 2,3,5-tri-iodobenzoic acid,    trinexapac-ethyl and uniconazole;-   N) Herbicides    -   acetamides selected from: acetochlor (N.1.1), alachlor,        butachlor, dimethachlor, dimethenamid (N.1.2), flufenacet        (N.1.3), mefenacet (N.1.4), metolachlor (N.1.5), metazachlor        (N.1.6), napropamide, naproanilide, pethoxamid, pretilachlor,        propachlor, thenylchlor;    -   amino acid derivatives selected from: bilanafos, glyphosate        (N.2.1), glufosinate (N.2.2), sulfosate (N.2.3);    -   aryloxyphenoxypropionates: clodinafop (N.3.1), cyhalofop-butyl,        fenoxaprop (N.3.2), fluazifop (N.3.3), haloxyfop (N.3.4),        metamifop, propaquizafop, quizalofop, quizalofop-P-tefuryl;    -   Bipyridyls: diquat, paraquat (N.4.1);    -   (thio)carbamates selected from: asulam, butylate, carbetamide,        desmedipham, dimepiperate, eptam (EPTC), esprocarb, molinate,        orbencarb, phenmedipham (N.5.1), prosulfocarb, pyributicarb,        thiobencarb, triallate;    -   cyclohexanediones selected from: butroxydim, clethodim (N.6.1),        cycloxydim (N.6.2), profoxydim (N.6.3), sethoxydim (N.6.4),        tepraloxydim (N.6.5), tralkoxydim;    -   dinitroanilines selected from: benfluralin, ethalfluralin,        oryzalin, pendimethalin (N.7.1), prodiamine (N.7.2), trifluralin        (N.7.3);    -   diphenyl ethers selected from: acifluorfen (N.8.1), aclonifen,        bifenox, diclofop, ethoxyfen, fomesafen, lactofen, oxyfluorfen;    -   hydroxybenzonitriles selected from: bomoxynil (N.9.1),        dichlobenil, ioxynil;    -   imidazolinones selected from: imazamethabenz, imazamox (N.10.1),        imazapic (N.10.2), imazapyr (N.10.3), imazaquin (N.10.4),        imazethapyr (N.10.5);    -   phenoxy acetic acids selected from: clomeprop,        2,4-dichlorophenoxyacetic acid (2,4-D) (N.11.1), 2,4-DB,        dichlorprop, MCPA, MCPA-thioethyl, MCPB, Mecoprop;    -   pyrazines selected from: chloridazon (N.11.1), flufenpyr-ethyl,        fluthiacet, norflurazon, pyridate;    -   pyridines selected from: aminopyralid, clopyralid (N.12.1),        diflufenican, dithiopyr, fluridone, fluroxypyr (N.12.2),        picloram (N.12.3), picolinafen (N.12.4), thiazopyr;    -   sulfonyl ureas selected from: amidosulfuron, azimsulfuron,        bensulfuron (N.13.1), chlorimuron-ethyl (N.13.2), chlorsulfuron,        cinosulfuron, cyclosulfamuron (N.13.3), ethoxysulfuron,        flazasulfuron, flucetosulfuron, flupyrsulfuron, foramsulfuron,        halosulfuron, imazosulfuron, iodosulfuron (N.13.4), mesosulfuron        (N.13.5), metazosulfuron, metsulfuron-methyl (N.13.6),        nicosulfuron (N.13.7), oxasulfuron, primisulfuron, prosulfuron,        pyrazosulfuron, rimsulfuron (N.13.8), sulfometuron,        sulfosulfuron, thifensulfuron, triasulfuron, tribenuron,        trifloxysulfuron, triflusulfuron (N.13.9), tritosulfuron,        1-((2-chloro-6-propyl-imidazo[1,2-b]pyridazin-3-yl)sulfonyl)-3-(4,6-dimethoxy-pyrimidin-2-yl)urea;    -   triazines: ametryn, atrazine (N.14.1), cyanazine, dimethametryn,        ethiozin, hexazinone (N.14.2), metamitron, metribuzin,        prometryn, simazine, terbuthylazine, terbutryn, triaziflam;    -   ureas selected from: chlorotoluron, daimuron, diuron (N.15.1),        fluometuron, isoproturon, linuron, methabenzthiazuron,        tebuthiuron;    -   other acetolactate synthase inhibitors selected from:        bispyribac-sodium, cloransulam-methyl, diclosulam, florasulam        (N.16.1), flucarbazone, flumetsulam, metosulam,        ortho-sulfamuron, penoxsulam, propoxycarbazone,        pyribambenz-propyl, pyribenzoxim, pyriftalid,        pyriminobac-methyl, pyrimisulfan, pyrithiobac, pyroxasulfone        (N.16.2), pyroxsulam;    -   others selected from: amicarbazone, aminotriazole, anilofos,        beflubutamid, benazolin, bencarbazone,benfluresate, benzofenap,        bentazone (N.17.1), benzobicyclon, bicyclopyrone, bromacil,        bromobutide, butafenacil, butamifos, cafenstrole, carfentrazone,        cinidon-ethyl (N.17.2), chlorthal, cinmethylin (N.17.3),        clomazone (N.17.4), cumyluron, cyprosulfamide, dicamba (N.17.5),        difenzoquat, diflufenzopyr (N.17.6), Drechslera monoceras,        endothal, ethofumesate, etobenzanid, fenoxasulfone,        fentrazamide, flumiclorac-pentyl, flumioxazin, flupoxam,        flurochloridone, flurtamone, indanofan, isoxaben, isoxaflutole,        lenacil, propanil, propyzamide, quinclorac (N.17.7), quinmerac        (N.17.8), mesotrione (N.17.9), methyl arsonic acid, naptalam,        oxadiargyl, oxadiazon, oxaziclomefone, pentoxazone, pinoxaden,        pyraclonil, pyraflufen-ethyl, pyrasulfotole, pyrazoxyfen,        pyrazolynate, quinoclamine, saflufenacil (N.17.10), sulcotrione        (N.17.11), sulfentrazone, terbacil, tefuryltrione, tembotrione,        thiencarbazone, topramezone (N.17.12),        (3-[2-chloro-4-fluoro-5-(3-methyl-2,6-dioxo-4-trifluoromethyl-3,6-dihydro-2H-pyrimidin-1-yl)-phenoxy]-pyridin-2-yloxy)-acetic        acid ethyl ester,        6-amino-5-chloro-2-cyclopropyl-pyrimidine-4-carboxylic acid        methyl ester,        6-chloro-3-(2-cyclopropyl-6-methyl-phenoxy)-pyridazin-4-ol,        4-amino-3-chloro-6-(4-chloro-phenyl)-5-fluoro-pyridine-2-carboxylic        acid,        4-amino-3-chloro-6-(4-chloro-2-fluoro-3-methoxy-phenyl)-pyridine-2-carboxylic        acid methyl ester, and        4-amino-3-chloro-6-(4-chloro-3-dimethylamino-2-fluoro-phenyl)-pyridine-2-carboxylic        acid methyl ester;-   O) Insecticides    -   organo(thio)phosphates selected from: acephate (O.1.1),        azamethiphos (O.1.2), azinphos-methyl (O.1.3), chlorpyrifos        (O.1.4), chlorpyrifos-methyl (O.1.5), chlorfenvinphos (O.1.6),        diazinon (O.1.7), dichlorvos (O.1.8), dicrotophos (O.1.9),        dimethoate (O.1.10), disulfoton (O.1.11), ethion (O.1.12),        fenitrothion (O.1.13), fenthion (O.1.14), isoxathion (O.1.15),        malathion (O.1.16), methamidophos (O.1.17), methidathion        (O.1.18), methyl-parathion (O.1.19), mevinphos (O.1.20),        monocrotophos (O.1.21), oxydemeton-methyl (O.1.22), paraoxon        (O.1.23), parathion (O.1.24), phenthoate (O.1.25), phosalone        (O.1.26), phosmet (O.1.27), phosphamidon (O.1.28), phorate        (O.1.29), phoxim (O.1.30), pirimiphos-methyl (O.1.31),        profenofos (O.1.32), prothiofos (O.1.33), sulprophos (O.1.34),        tetrachlorvinphos (O.1.35), terbufos (O.1.36), triazophos        (O.1.37), trichlorfon (O.1.38);    -   carbamates selected from: alanycarb (O.2.1), aldicarb (O.2.2),        bendiocarb (O.2.3), benfuracarb (O.2.4), carbaryl (O.2.5),        carbofuran (O.2.6), carbosulfan (O.2.7), fenoxycarb (O.2.8),        furathiocarb (O.2.9), methiocarb (O.2.10), methomyl (O.2.11),        oxamyl (O.2.12), pirimicarb (O.2.13), propoxur (O.2.14),        thiodicarb (O.2.15), triazamate (O.2.16);    -   pyrethroids selected from: allethrin (O.3.1), bifenthrin        (0.3.2), cyfluthrin (O.3.3), cyhalothrin (O.3.4), cyphenothrin        (O.3.5), cypermethrin (O.3.6), alpha-cypermethrin (O.3.7),        beta-cypermethrin (O.3.8), zeta-cypermethrin (O.3.9),        deltamethrin (O.3.10), esfenvalerate (O.3.11), etofenprox        (O.3.11), fenpropathrin (O.3.12), fenvalerate (O.3.13),        imiprothrin (O.3.14), lambda-cyhalothrin (O.3.15), permethrin        (O.3.16), prallethrin (O.3.17), pyrethrin I and II (O.3.18),        resmethrin (O.3.19), silafluofen (O.3.20), tau-fluvalinate        (O.3.21), tefluthrin (O.3.22), tetramethrin (O.3.23),        tralomethrin (O.3.24), transfluthrin (O.3.25), profluthrin        (O.3.26), dimefluthrin (O.3.27);    -   insect growth regulators selected from: a) chitin synthesis        inhibitors: benzoylureas: chlorfluazuron (O.4.1), cyramazin        (O.4.2), diflubenzuron (O.4.3), flucycloxuron (O.4.4),        flufenoxuron (O.4.5), hexaflumuron (O.4.6), lufenuron (O.4.7),        novaluron (O.4.8), teflubenzuron (O.4.9), triflumuron (O.4.10);        buprofezin (O.4.11), diofenolan (O.4.12), hexythiazox (O.4.13),        etoxazole (O.4.14), clofentazine (O.4.15); b) ecdysone        antagonists: halofenozide (O.4.16), methoxyfenozide (O.4.17),        tebufenozide (O.4.18), azadirachtin (O.4.19); c) juvenoids:        pyriproxyfen (O.4.20), methoprene (O.4.21), fenoxycarb        (O.4.22); d) lipid biosynthesis inhibitors: spirodiclofen        (O.4.23), spiromesifen (O.4.24), spirotetramat (O.4.24);    -   nicotinic receptor agonists/antagonists compounds selected from:        clothianidin (O.5.1), dinotefuran (O.5.2), flupyradifurone        (O.5.3), imidacloprid (O.5.4), thiamethoxam (O.5.5), nitenpyram        (O.5.6), acetamiprid (O.5.7), thiacloprid (O.5.8),        1-2-chloro-thiazol-5-ylmethyl)-2-nitrimino-3,5-dimethyl-[1,3,5]triazinane        (O.5.9);    -   GABA antagonist compounds selected from: endosulfan (O.6.19,        ethiprole (O.6.2), fipronil (O.6.3), vaniliprole (O.6.4),        pyrafluprole (O.6.5), pyriprole (O.6.6),        5-amino-1-(2,6-dichloro-4-methyl-phenyl)-4-sulfinamoyl-1H-pyrazole-3-carbothioic        acid amide (O.6.7);    -   macrocyclic lactone insecticides selected from: abamectin        (O.7.1), emamectin (O.7.2), milbemectin (O.7.3), lepimectin        (O.7.4), spinosad (O.7.5), spinetoram (O.7.6);    -   mitochondrial electron transport inhibitor (METI) I acaricides        selected from: fenazaquin (O.8.1), pyridaben (O.8.2),        tebufenpyrad (O.8.3), tolfenpyrad (O.8.4), flufenerim (O.8.5);    -   METI II and III compounds: acequinocyl (O.9.1), fluacyprim        (O.9.2), hydramethylnon (O.9.3);    -   Uncoupler: chlorfenapyr (O.10.1);    -   oxidative phosphorylation inhibitors selected from: cyhexatin        (O.11.1), diafenthiuron (O.11.2), fenbutatin oxide (O.11.3),        propargite (O.11.4);    -   moulting disruptor compound: cryomazine (O.12.1);    -   mixed function oxidase inhibitor: piperonyl butoxide (O.13.1);    -   sodium channel blockers selected from: indoxacarb (O.14.1),        metaflumizone (O.14.2);    -   ryanodine receptor inhibitors selected from: chlorantraniliprole        (O.15.1), cyantraniliprole (O.15.2), flubendiamide (O.15.3),        N-[4,6-dichloro-2-[(diethyl-lambda-4-sulfanylidene)carba-moyl]-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide        (O.15.4);        N-[4-chloro-2-[(diethyl-lambda-4-sulfanylidene)carbamoyl]-6-Methyl-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyppyrazole-3-carboxamide        (O.15.5);        N-[4-chloro-2-[(di-2-propyl-lambda-4-sulfanylidene)carbamoyl]-6-methyl-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoro-methyl)pyrazole-3-carboxamide        (O.15.6);        N-[4,6-dichloro-2-[(di-2-propyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyppyrazole-3-carboxamide        (O.15.7);        N-[4,6-dichloro-2-[(diethyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2-(3-chloro-2-pyridyl)-5-(difluoromethyl)pyrazole-3-carboxamide        (O.15.8);        N-[4,6-dibromo-2-[(di-2-propyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyppyrazole-3-carboxamide        (O.15.9);        N-[4-chloro-2-[(di-2-propyl-lambda-4-sulfanylidene)carbamoyl]-6-cyano-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide        (O.15.10);        N-[4,6-dibromo-2-[(diethyl-lambda-4-sulfanylidene)carbamoyl]-phenyl]-2-(3-chloro-2-pyridyl)-5-(trifluoromethyl)pyrazole-3-carboxamide        (O.15.11);    -   others selected from: benclothiaz (O.16.1), bifenazate (O.16.2),        artap (O.16.3), flonicamid (O.16.4), pyridalyl (O.16.5),        pymetrozine (O.16.6), sulfur (O.16.7), thiocyclam (O.16.8),        cyenopyrafen (O.16.9), flupyrazofos (O.16.10), cyflumetofen        (O.16.11), amidoflumet (O.16.12), imicyafos (O.16.13),        bistrifluron (O.16.14), pyrifluquinazon (O.16.15) and        1,1′-[(3S,4R,4aR,6S,6aS,12R,12aS,12bS)-4-[[(2-cyclopropylacetypoxy]methyl]-1,3,4,4a,5,6,6a,12,12a,12b-decahydro-12-hydroxy-4,6a,12b-trimethyl-11-oxo-9-(3-pyridinyl)-2H,11H-naphtho[2,1-b]pyrano[3,4-e]pyran-3,6-diyl]        cyclopropaneacetic acid ester (O.16.16).

According to one embodiment, the mixtures comprise at least onemicroorganism of the genus Bacillus seleted from B. amyloliquefaciensAP-136 (NRRL B-50330; NRRL B-50614), B. amyloliquefaciens AP-188 (NRRLB-50331; NRRL B-50615), B. amyloliquefaciens AP-218 (NRRL B-50618), B.amyloliquefaciens AP-219 (NRRL B-50332, NRRL B-50619), B.amyloliquefaciens AP-295 (NRRL B-50333, NRRL B-50620) B. mojavensisAP-209 (NRRL B-50616), and B. solisalsi AP-217 (NRRL B-50617) (which areherein also referred to as AP-136, AP-188, AP-218, AP-219, AP-295,AP-209 and AP-217, respectively); and at least one pesticide II in asynergistically effective amount.

The invention also relates to a method for controlling phytopathogenicharmful fungi using mixtures of at least one microorganism selected fromAP-136, AP-188, AP-218, AP-219, AP-295, AP-209 and AP-217 and and atleast one pesticide II and to the use of microorganisms I and pesticidesII for preparing such mixtures, and to compositions comprising thesemixtures and seed comprising these mixtures or coated with this thismixture.

Moreover, we have found that simultaneous, that is joint or separate,application of at least one microorganism I and a pesticide II orsuccessive application of at least one microorganism I and of apesticide II allows better control of harmful fungi than is possiblewith the individual components alone (synergistic mixtures).

When applying the at least one microorganism I and a pesticide IIsequentially the time between both applications may vary e.g. between 2hours and 7 days. Also a broader range is possible ranging from 0.25hour to 30 days, preferably from 0.5 hour to 14 days, particularly from1 hour to 7 days or from 1.5 hours to 5 days, even more preferred from 2hours to 1 day. Preferably, the at least one microorganism I is appliedas last treatment.

Component 1) in the mixtures embraces not only the isolated, purecultures of at least one microorganism I as defined herein, but also aits cell-free extract having pesticidal activity, preferably aketone-based extract, its suspensions in a whole broth culture or as ametabolite-containing supernatant or a purified metabolite obtained froma whole broth culture of the microorganism or microorganism strain.

As used herein, “whole culture broth” refers to a liquid culture of amicroorganism containing vegetative cells and/or spores suspended in theculture medium and optionally metabolites produced by the respectivemicroorganism.

As used herein, “strain” refers to isolate or a group of isolatesexhibiting phenotypic and/or genotypic traits belonging to the samelineage, distinct from those of other isolates or strains of the samespecies.

According to a further embodiment, component 1) is at least onemicroorganism selected from AP-136, AP-188, AP-218, AP-219, AP-295,AP-209 and AP-217, and a cell-free extract of the aforementionedstrains. According to a further embodiment, component 1) is at least onemicroorganism selected from AP-136, AP-188, AP-218, AP-219, AP-295,AP-209 and AP-217. According to a further embodiment, component 1) is atleast one microorganism selected from AP-136, AP-188, AP-218, AP-219,AP-295, AP-209 and AP-217 in a whole browth. According to a furtherembodiment, component 1) is at least one microorganism selected fromAP-136, AP-188, AP-218, AP-219, AP-295, AP-209 and AP-217 in a dormantform. According to a further embodiment, component 1) is at least onemicroorganism selected from AP-136, AP-188, AP-218, AP-219, AP-295,AP-209 and AP-217 in the form of spores.

The microorganisms AP-136, AP-188, AP-218, AP-219, AP-295, AP-209 andAP-217 can be cultivated e.g. using Potato dextrose agar (PDA) underaerobvic growth conditions at about 28° C. In large liquid cultures,aeriation may be necessary. The bacterial cells (vegatitive cells andspores) can be washed and concentrated (e.g. by centrifugation at roomtemperature for about 15 min at 7000×g). To produce a dry formulation,bacterial cells, preferably spores were suspended in a suitable drycarrier (e.g. clay). To produce a liquid formulation, cells, preferablyspores, can be re-suspended in a suitable liquid carrier (e.g.water-based) to the desired spore density. The spore density number ofspores per mL can be determined by identifying the number ofcolony-forming units (CFU) on agar medium e.g. potato dextrose agarafter incubation for several days at 28° C. The microorganism I aregenerally active in temperatures between 5° C. and 50° C., preferablybetween 15° C. and 35° C.

According to a further embodiment, component 1) is at least onemicroorganism I selected from Bacillus amyloliquefaciens AP-136 (NRRLB-50330; NRRL B-50614), B. amyloliquefaciens AP-188 (NRRL B-50331; NRRLB-50615), B. amyloliquefaciens AP-218 (NRRL B-50618), B.amyloliquefaciens AP-219 (NRRL B-50332, NRRL B-50619), and B.amyloliquefaciens AP-295 (NRRL B-50333, NRRL B-50620); in particular B.amyloliquefaciens AP-188.

According to a further embodiment, component 1) is at least onemicroorganism I selected from B. mojavensis AP-209 (NRRL B-50616) and B.solisalsi AP-217 (NRRL B-50617).

According to a further embodiment, component 2) is selected fromBradyrhizobium japonicum, B. elkanii, Bradyrhizobium spp.,Bradyrhizobium sp. (Arachis), Bradyrhizobium sp. (Vigna), B.liaoningense, B. lupine; Azospirillum brasilense, A. amazonense, A.lipoferum, A. irakense, A. halopraeferens; Delftia acidovorans, Glomusintraradices; Mesorhizobium spp., Mesorhizobium ciceri, M. huakii, M.loti; Rhizobium leguminosarum bv. phaseoli, R. leguminosarum bv.trifolii, R. leguminosarum bv. viciae, R. tropici, Sinorhizobiummellloti; Bacillus amyloliquefaciens, B. amyloliquefaciens ssp.plantarum, B. firmus, B. pumilus, B. subtilis, B. simplex, B.megaterium, B. altitudinis, B. mojavensis, B. mycoides, B. solisalsi,Burkholderia spp., Coniothyrium minitans, Muscodor albus, Paecilomyceslilacinus, Paenibacillus alvei, Pasteuria nishizawa, Pasteuria usgae,Penicillium bilaiae, Pseudomonas fluorescens, Pseudomonas putida;abscisic acid, jasmonic acid, its salts and derivatives thereof,cis-jasmone, methyl jasmonate; harpin protein.

According to a further embodiment, component 2) is selected fromBradyrhizobium japonicum, B. elkanii, Azospinflum brasilense; Bacillusamyloliquefaciens, B. amyloliquefaciens ssp. plantarum, B. firmus, B.pumilus, B. subtilis, B. simplex, B. megaterium; Burkholderia spp.,Coniothyrium minitans, Muscodor albus, Paecilomyces lilacinus,Paenibacillus alvei, Penicillium bilaiae, Pasteuria nishizawa;cis-jasmone, methyl jasmonate and harpin protein.

According to a further embodiment, the mixtures comprise as component 2)at least one pesticide II selected from Bradyrhizobium japonicum, B.elkanii, Azospirillum brasilense; cis-jasmone, methyl jasmonate andharpin protein.

According to a further embodiment, component 2) is selected fromBacillus firmus, B. pumilus, Burkholderia spp., Muscodor albus andPaecilomyces lilacinus. These mixture are especially effective forcontrol of nematodes.

According to a further embodiment, the mixture comprise in addition tocomponent 1) and component 2) a further active component 3) which isselected from methyl jasmonate, cis-jasmone and harpin, provided thatcomponent 2) is different from component 3).

The biopesticides from group L) of pesticides II, their preparation andtheir pesticidal activity e. g. against harmful fungi or insects areknown (e-Pesticide Manual V 5.2 (ISBN 978 1 901396 85 0) (2008-2011);http://www.epa.gov/opp00001/biopesticides/, see product lists therein;http://www.omri.org/omri-lists, see lists therein; Bio-PesticidesDatabase BPDB http://sitem.herts.ac.uk/aeru/bpdb/, see A to Z linktherein).

The biopesticides from group L1) and/or L2) may also have insecticidal,acaricidal, molluscidal, pheromone, nematicidal, plant stress reducing,plant growth regulator, plant growth promoting and/or yield enhancingactivity. The biopesticides from group L3) and/or L4) may also havefungicidal, bactericidal, viricidal, plant defense activator, plantstress reducing, plant growth regulator, plant growth promoting and/oryield enhancing activity. The biopesticides from group L5) and/or L6)may also have fungicidal, bactericidal, viricidal, plant defenseactivator, insecticidal, acaricidal, molluscidal, pheromone and/ornematicidal activity.

Many of these biopesticides have been deposited under deposition numbersmentioned herein (the prefices refer to the acronym of the respectiveculture collection), are referred to in literature, registered and/orare commercially available: aluminium silicate (Screen™ Duo from CertisLLC, USA), Agrobacterium radiobacter K1026 (e. g. NoGall® from BASFAgricultural Specialties Pty Ltd, Australia), A. radiobacter K84 (Nature280, 697-699, 1979; e. g. GallTroll® from AG Biochem, Inc., C, USA),Ampelomyces quisqualis M-10 (e. g. AQ 10® from Intrachem Bio GmbH & Co.KG, Germany), Ascophyllum nodosum (Norwegian kelp, Brown kelp) extractor filtrate (e. g. ORKA GOLD from BASF Agricultural Specialities (Pty)Ltd., South Africa; or Goemar® from Laboratoires Goemar, France),Aspergillus flavus NRRL 21882 isolated from a peanut in Georgia in 1991by USDA, National Peanut Research Laboratory (e. g. in Afla-Guard® fromSyngenta, CH), mixtures of Aureobasidium pullulans DSM 14940 and DSM14941 (e. g. blastospores in Blossom Protect® from bio-ferm GmbH,Germany), Azospirillum amazonense SpY2 (DN: BR 11140; Proc. 9^(th) Int.and 1^(st) Latin American PGPR meeting, Quimara, Medellín, Colombia2012, p. 60, ISBN 978-958-46-0908-3), A. brasllense AZ39 (also called Az39; INTA Az-39; Eur. J. Soil Biol 45(1), 28-35, 2009), A. brasilense XOH(e. g. AZOS from Xtreme Gardening, USA or RTI Reforestation TechnologiesInternational; USA), A. brasilense BR 11002 (Proc. 9^(th) Int. and1^(st) Latin American PGPR meeting, Quimara, Medellín, Colombia 2012, p.60, ISBN 978-958-46-0908-3), A. brasilense Sp245 (BR 11005; e. g. inGELFIX Gramíneas from BASF Agricultural Specialties Ltd., Brazil), A.brasilense strains Ab-V5 and Ab-V6 (e. g. in AzoMax from Novozymes BioAgProdutos papra Agricultura Ltda., Quattro Barras, Brazil orSimbioseMaíz® from Simbiose-Agro, Cruz Alta, RS, Brazil; Plant Soil 331,413-425, 2010), A. lipoferum BR 11646 (Sp31) (Proc. 9^(th) Int. and1^(st) Latin American PGPR meeting, Quimara, Medellín, Colombia 2012, p.60), Bacillus altitudinis 41KF2b (DSM 21631; Int. J. Syst. Evol.Microbiol. 56(7), 1465-1473, 2006), Bacillus amyloliquefaciens strainsAP-136 (NRRL B-50614 and B-50330), AP-188 (NRRL B-50615 and B-50331),AP-218 (NRRL B-50618), AP-219 (NRRL B-50619 and B-50332), and AP-295(NRRL B-50620 and B-50333) all known from U.S. Pat. No. 8,445,255; B.amyloliquefaciens IT-45 (CNCM 1-3800) (e. g. Rhizocell C from ITHEC,France), B. amyloliquefaciens IN937a (J. Microbiol. Biotechnol. 17(2),280-286, 2007; e. g. BioYield® from Gustafson LLC, TX, USA), B.amyloliquefaciens spp. plantarum D747 (US 20130236522 A1; FERM BP-8234;e. g. Double Nickel™ 55 WDG or Double Nickel™ LC from Certis LLC, USA),B. amyloliquefaciens spp. plantarum FZB24 isolated from plantpathogen-infested soil of a sugar beet field in Brandenburg, Germany(also called SB3615; DSM ID 96-2; J. Plant Dis. Prot. 105, 181-197,1998; e. g. Taegro® from Novozyme Biologicals, Inc., USA), B.amyloliquefaciens spp. plantarum SB3615vPPI being a phage-resistantvariant of FZB24 (MRRL B-50349; US 2011/023045 A1; from NovozymeBiologicals, Inc., USA), B. amyloliquefaciens ssp. plantarum FZB42isolated from plant pathogen-infested soil of a sugar beet field inBrandenburg, Germany (J. Plant Dis. Prot. 105, 181-197, 1998; DSM 23117;e. g. RhizoVital® 42 from AbiTEP GmbH, Berlin, Germany), B.amyloliquefaciens ssp. plantarum GB03 (also called GBO3; ATCC SD-1397;Phytopathol. 86(11), S36, 1996; e. g. Kodiak® or BioYield® fromGustafson, Inc., USA; or Companion® from Growth Products, Ltd., WhitePlains, N.Y. 10603, USA), B. amyloliquefaciens ssp. plantarum MB1600also referred to as 1430 (NRRL B-50595; Int. J. Microbiol. Res. 3(2)(2011), 120-130; US 2012/0149571 A1; e. g. Integral®, Subtilex® NG fromBASF Corp., USA), B. amyloliquefaciens spp. plantarum TJ1000 (alsocalled 1BE; CA 2471555 A1; ATCC BAA-390; e. g. QuickRoots™ from TJTechnologies, Watertown, S. Dak., USA), B. cereus CNCM 1-1562 (U.S. Pat.No. 6,406,690), B. chitinosporus AQ746 isolated from roots inSaskatchewan, Canada (NRRL B-21618; U.S. Pat. No. 5,733,544; AgraQuestnow Bayer CropScience LP, USA), B. firmus CNCM 1-1582 (WO 2009/126473,WO 2009/124707, U.S. Pat. No. 6,406,690; e. g. Votivo® from BayerCropScience LP, USA), B. megaterium strains H491 (NRRL B-50769), M018(NRRL B-50770) and J142 (NRRL B-50771) all known from US 2014/0051571 A1from Marrone BioInnovations, Inc., USA; B. mojavensis AP-209 (NRRLB-50616; U.S. Pat. No. 8,445,255), B. mycoides AQ726 (NRRL B-21664; U.S.Pat. No. 5,906,818; from Bayer Crop Science, Germany), B. mycoidesstrain J (e.g. BmJ WG from Certis, USA against potato virus Y), B.pumilus GB34 (ATCC 700814; e. g. YieldShield® from Gustafson LLC, TX,USA), B. pumilus GHA 180 isolated from apple tree rhizosphere in Mexico(IDAC 260707-01; e. g. in PRO-MIX® BX from Premier Horticulture, 1,avenue Premier, Rivie're-du-Loup, Quebec, Canada G5R6C1), B. pumilusKFP9F (NRRL B-50754; WO 2014/029697; e. g. BAC-UP or FUSION-P from BASFAgricultural Specialities (Pty) Ltd., South Africa), B. pumilus INR-7otherwise referred to as BU-F22 and BU-F33 (NRRL B-50185, NRRL B-50153;U.S. Pat. No. 8,445,255), B. pumilus QST 2808 (NRRL B-30087; e. g.Sonata® or Ballad® Plus from AgraQuest Inc., USA), B. solisalsi AP-217(NRRL B-50617; U.S. Pat. No. 8,445,255), B. subtilis CX-9060 (FederalRegister 77(7), 1633-1637; by Certis U.S.A., L.L.C.), B. subtilis FB17also called UD 1022 or UD10-22 isolated from red beet roots in NorthAmerica (ATCC PTA-11857; System. Appl. Microbiol. 27, 372-379, 2004; US2010/0260735; WO 2011/109395); B. subtilis GB07 (Phytopathol. 86(11),S36, 1996; Epic® from Gustafson, Inc., USA), B. subtilis QST-713isolated from a California peach orchard in 1995 (NRRL B-21661; e. g.Rhapsody®, Serenade® MAX or Serenade® ASO from AgraQuest Inc., USA), B.thuringiensis ssp. aizawai ABTS-1857 (also called ABG-6346; ATCCSD-1372; e. g. XenTari® from BioFa AG, Munsingen, Germany), B. t. ssp.aizawai SAN 401 I, ABG-6305 (WO 2013/087709); Bacillus t. ssp.israelensis AM65-52 of Serotype H-14 (ATCC SD-1276; e. g. VectoBac® fromValent BioSciences, IL, USA), Bacillus thuringiensis ssp. kurstaki SB4(NRRL B-50753; e. g. Beta Pro® from BASF Agricultural Specialities (Pty)Ltd., South Africa), B. t. ssp. kurstaki ABTS-351 identical to HD-1(ATCC SD-1275; e. g. Dipel® DF from Valent BioSciences, IL, USA), B. t.ssp. kurstaki EG 2348 (NRRL B-18208; e. g. Lepinox® or Rapax® from CBC(Europe) S.r.I., Italy), B. t. ssp. tenebrionis DSM 2803 of Serotype H8a, 8b (identical to NRRL B-15939; EP 0 585 215 B1; Mycogen Corp.), B.t. ssp. tenebrionis NB-125 (also referred to as SAN 418 I or ABG-6479;EP 0 585 215 B1; DSM 5526; former production strain of Novo-Nordisk), B.t. ssp. tenebrionis NB-176 (or NB-176-1; a gamma-irridated, inducedhigh-yielding mutant of strain NB-125; EP 585 215 B1; DSM 5480; e. g.Novodor® from Valent BioSciences, Switzerland), Beauveria bassiana JW-1(ATCC 74040; e. g. Naturalis® from CBC (Europe) S.r.I., Italy), B.bassiana DSM 12256 (US 200020031495; e. g. BioExpert® SC from LiveSytems Technology S.A., Colombia), B. bassiana GHA (ATCC 74250; e. g.BotaniGard® 22WGP from Laverlam Int. Corp., USA), B. bassiana PPRI 5339(ARSEF 5339; NRRL 50757; e. g. BroadBand® from BASF AgriculturalSpecialities (Pty) Ltd., South Africa), B. brongniartii for control ofcockchafer (J. Appl. Microbiol. 100(5),1063-72, 2006; e. g. Melocont®from Agrifutur, Agrianello, Italy), Bradyrhizobium sp. (e. g. Vault®from BASF Corp., USA), B. sp. (Arachis) CB1015 presumably originallycollected in India (IITA 1006, USDA 3446; from Australian InoculantsResearch Group; http://www.qaseeds.com.au/inoculant_applic.php). B. sp.(Arachis) strains deposited at SEMIA and known from FEMS Microbiol.Letters 303(2), 123-131, 2010; Revista Brasileira de Ciencia do Solo35(3), 739-742, 2011, ISSN 0100-0683: SEMIA 6144, SEMIA 6462 (BR 3267)and SEMIA 6464 (BR 3262); B. sp. (Vigna) PNLO1 (Bisson and Mason, Apr.29, 2010, Project report, Worcester Polytechnic Institute, Worcester,Mass., USA:http://www.wpi.edu/Pubs/E-project/Available/E-project-042810-163614/; e.g. Vault® Peanut Liquid from BASF Corp., USA), B. elkanii SEMIA 587(Appl. Environ. Microbiol. 73(8), 2635, 2007; e. g. GELFIX 5 from BASFAgricultural Specialties Ltd., Brazil), B. elkanii SEMIA 5019 (=29W;Appl. Environ. Microbiol. 73(8), 2635, 2007; e. g. GELFIX 5 from BASFAgricultural Specialties Ltd., Brazil), B. elkanii USDA 76, B. elkaniiUSDA 94B. elkanii USDA 3254, B. elkanii U-1301 and U-1302 (e. g.Nitragin® Optimize from Novozymes Bio As S.A., Brazil, or Nlitrasec forsoybean from LAGE y Cia, Brazil), B. japonicum (e. g. VAULT® from BASFCorp., USA), B. japonicum 532c isolated from Wisconsin field (Nitragin61A152; Can. J. Plant. Sci. 70, 661-666, 1990; e. g. in Rhizoflo®,Histick®, Hicoat® Super from BASF Agricultural Specialties Ltd.,Canada), B. japonicum E-109 variant of strain USDA 138 (INTA E109, SEMIA5085; Eur. J. Soil Biol. 45, 28-35, 2009; Biol. Fertil. Soils 47, 81-89,2011), B. japonicum G49 (MSDJ G49; C. R. Acad. Agric. Fr. 73, 163-171,1987); B. japonicum strains deposited at SEMIA known from Appl. Environ.Microbiol. 73(8), 2635, 2007: SEMIA 566 isolated from North Americaninoculant in 1966 and used in Brazilian commercial inoculants from 1966to 1978, SEMIA 586 originally isolated in Maryland, USA, in 1961 butreceived from Australia in 1966 and used in Brazilian inoculants in 1977(CB 1809, USDA 136, Nitragin 61A136, RCR 3407), SEMIA 5079 a naturalvariant of SEMIA 566 used in commercial inoculants since 1992 (CPAC 15;e. g. GELFIX 5 or ADHERE 60 from BASF Agricultural Specialties Ltd.,Brazil), B. japonicum SEMIA 5080 a natural variant of SEMIA 586 used incommercial inoculants since 1992 (CPAC 7; e. g. GELFIX 5 or ADHERE 60from BASF Agricultural Specialties Ltd., Brazil); B. japonicum TA-11(TA11 NOD+) (NRRL B-18466; U.S. Pat. No. 5,021,076; Appl. Environ.Microbiol. 56, 2399-2403, 1990; e. g. VAULT® NP, from BASF Corp., USA),B. japonicum strains deposited at USDA known from U.S. Pat. No.7,262,151 and Appl. Environ. Microbiol. 60, 940-94, 1994: USDA 3isolated from Glycine max in Virginia (USA) in 1914, USDA 31 (=Nitragin61A164) od Serogroup 31 isolated from Glycine max in Wisconsin (USA) in1941, USDA 76 isolated from plant passage of strain USDA 74 (Serogroup76) which has been isolated from G. max in California (USA) in 1956,USDA 110 (=IITA 2121, SEMIA 5032, RCR 3427, ARS 1-110 and Nitragin61A89; Serogroup 110) isolated from G. max in Florida in 1959, USDA 121isolated from G. max in Ohio (USA) in 1965 (Crop Science 26(5), 911-916,1986); B. japonicum WB74 (e. g. Eco-Rhiz Soya from Plant Health Products(Pty) Ltd, South Africa; or Soybean inoculant from Stimuplant CC, SouthAfrica), B. lupini LL13 isolated from Lupinus iuteus nodules from Frenchsoils (deposited at INRA, France; http://agriculture.gouv.fr/IMG/pdf/ch20060216.pdf), B. lupini strains from Australia andknown from Palta J. A., Berger J. B. (eds), Proceed. 12^(th)International Lupin Conference, 14-18 Sep. 2008, Fremantle, WesternAustralia, International Lupin Association, Canterbury, New Zealand,47-50,http://www.lupins.org/pdf/conference/2008/Agronomy%20and%20Production/John%20Howieson%20and%20G%20OHara.pdf;Appl. Environ. Microbiol. 71, 7041-7052, 2005; Australian J. Exp.Agricult. 36(1), 63-70, 1996: strains WU425 isolated in Esperance,Western Australia from a non-Australian legume Ornithopus compressus,WSM471 isolated from Ornithopus pinnatus in Oyster Harbour, WesternAustralia, and WSM4024 isolated from lupins in Australia by CRS during a2005 survey; Burkholderia sp. A396 (NRRL B-50319; WO 2013/032693;Marrone Bio Innovations, Inc., USA), Candida oleophila I-182 (NRRLY-18846; Phytoparasitica 23(3), 231-234, 1995; e. g. Aspire® from EcogenInc., USA), C. oleophila strain O (NRRL Y-2317; Biological Control 51,403-408, 2009), Candida saitoana (e. g. Biocure® [in mixture withlysozyme] and BioCoat® from Micro Flo Company, USA (BASF SE) andArysta), chitosan (e. g. Armour-Zen® from BotriZen Ltd., NZ),Clonostachys rosea f. catenulate (also named Gliocladium catenulatum)J1446 isolated from Finnish field soil (NJF seminar No 389: Pest,disease and weed management in strawberry; Finland 8-9. Nov. 2006 in NJFReport 2(10), 15-15, 2006; DSM 9212; e. g. Primastop® or Prestop® fromVerdera Oy, Finland), Chromobacterium subtsugae PRAA4-1 isolated fromsoil under an eastern hemlock (Tsuga canadensis) in the CatoctinMountain region of central Maryland (NRRL B-30655; e. g. Grandevo® fromMarrone Bio Innovations, USA), Coniothyrium minitans CON/M/91-08 (WO1996/021358; DSM 9660; e. g. Contans® WG, Intercept® WG from ProphytaBiologischer Pflanzenschutz GmbH, Germany), Cryphonectria parasitica(hypovirulent strains; Microbiol. Reviews 56(4), 561-576, 1992; e. g.product Endothia parasitica from CNICM, France), Cryptococcus albidus(e. g. YIELD PLUS® from Anchor Bio-Technologies, South Africa),Cryptophlebia leucotreta granulovirus (CrleGV) (e. g. CRYPTEX fromAdermatt Biocontrol, Switzerland), Cydia pomonella granulovirus (CpGV)V03 (DSM GV-0006; e. g. Madex® Max from Andermatt Biocontrol,Switzerland), CpGV V22 (DSM GV-0014; e. g. Madex® Twin from AdermattBiocontrol, Switzerland), Delftia acidovorans RAY209 (ATCC PTA-4249; WO2003/57861; e. g. BioBoost® from Brett Young, Winnipeg, Canada),Dilophosphora alopecuri (FarmNote 396, February 2010, Department ofAgriculture and Food, Government of Western Australia; e.g. Twist Fungusfrom BASF Agricultural Specialties Pty Ltd, Australia), Ecklonia maxima(kelp) extract (J. Ecological Engineering 14(1), 48-52, 2013; e. g.KELPAK SL from Kelp Products Ltd, South Africa), Flavobacterium sp. H492(ATCC B-505584; WO 2013/138398; e. g. MBI-302 from Marrone BioInnovations, USA for soyean cyst nematode control), formononetin (U.S.Pat. No. 5,002,603; e. g. Myconate® from Plant Health Care plc, U.K.),Fusarium oxysporum Fo47 (non-pathogenic strain isolated from asuppressive soil located at Châteaurenard, France; Appl. Environ.Microbiol 68(8), 4044-4060, 2002; Fusaclean® from Natural PlantProtection, N.P.P. (société anonyme) Route d′Artix F-64150 Nogueres,France), F. oxysporum 251/2RB (Prevention Today Vol. 2, n. 1-2, 47-62,2006; e. g. Biofox® C from S.I.A.P.A., Italy); Glomus intraradices (e.g. Myc® 4000 from ITHEC, France), Glomus intraradices RTI-801 (e. g.MYKOS from Xtreme Gardening, USA or RTI Reforestation TechnologiesInternational; USA), grapefruit seeds and pulp extract (e. g. BC-1000from Chemie S.A., Chile), harpin (alpha-beta) protein (Science 257,85-88, 1992; e. g. Messenger™ or HARP-N-Tek from Plant Health Care plc,U.K.), Helicoverpa armigera nucleopolyhedrovirus (HearNPV) (J.Invertebrate Pathol. 107, 112-126, 2011; e. g. Helicovex® from AdermattBiocontrol, Switzerland), Heterorhabditis bacteriophora (e. g. Nemasys®G from BASF Agricultural Specialities Limited, UK), Isaria fumosoroseaApopka-97 (ATCC 20874; Biocontrol Science Technol. 22(7), 747-761, 2012;e. g. PFR-97™ or PreFeRal® from Certis LLC, USA), I. fumosorosea FE 9901(ARSEF 4490; Biocontrol Science Technol. 22(7), 747-761, 2012; e. g.blastospores in NoFly™ WP from Natural Industries, Inc., Houston, Tex.,USA or from Novozymes, U.S.A.), cis-jasmone (U.S. Pat. No. 6,890,525;U.S. Pat. No. 8,221,736; Plant Bioscience Limited, Norwich, U.K.),laminarin (e. g. in Vacciplant® from Laboratoires Goemar, St. Malo,France or Stahler SA, Switzerland), Lecanicillium longisporum KV42 andKV71 (e. g. Vertalec® from Koppert BV, Netherlands), L. muscarium Ve6(also called KV01; IMI 19-79, CABI 268317, CBS 102071, ARSEF 5128; e. g.Mycotal® from Koppert BV, Netherlands), Lysobacter antibioticus 13-1(Biological Control 45, 288-296, 2008), L. antibioticus HS124 (Curr.Microbiol. 59(6), 608-615, 2009), L. enzymogenes 3.1T8 (Microbiol. Res.158, 107-115, 2003; Biological Control 31(2), 145-154, 2004);Mesorhizobium spp. strains known from Soil Biol. Biochem. 36(8),1309-1317, 2004; Plant and Soil 348(1-2), 231-243, 2011: M. sp. WSM1271collected in Sardinia, Italy, from plant host Biserrula pelecinus, M.sp. WSM 1497 collected in Mykonos, Greece, from Biserrula pelecinus,Mesorhizobium ciceri CC1192 collected in Israel from Cicer arietinumnodules (UPM 848, CECT 5549; Can. J. Microbiol. 48, 279-284, 2002; fromHorticultural Research Station, Gosford, Australia), M. huakuii HN3015isolated from Astralagus sinicus in a rice-growing field of SouthernChina (World J. Microbiol. Biotechn. 23(6), 845-851, 2007, ISSN0959-3993), M. loti CC829 isolated from L. ulginosus nodules in USA (NZP2012; commerical inoculant for Lotus pedunculatus and L. ulginosus inAustralia), and M. loti SU343 isolated from host nodules in USA(commercial inoculant for Lotus corniculatus in Australia); Metarhiziumanisopliae FI-1045 (AGAL V10/0104285; WO 2012/018266; e. g. Biocane®from BASF Agricultural Specialties Pty Ltd, Australia), M. anisopliaevar. anisopliae F52 also called 275 or V275 (DSM 3884, ATCC 90448; e. g.Met52® Novozymes Biologicals BioAg Group, Canada), M. anisopliae ICIPE69 isolated from a soil sample obtained from the Democratic Republic ofCongo (DRC) and using the Galleria bait method in 1990 (e. g.Metathripol from ICIPE, Nairobe, Kenya), M. anisopliae var. acridum IMI330189 isolated from Ornithacris cavroisi Niger (NRRL 50758; e. g. GreenMuscle® from BASF Agricultural Specialities (Pty) Ltd., South Africa),M. a. var. acridum F1-985 isolated from a spur-throated locust,Austracris guttulosa (Walker), near Rockhampton, Queensland, Australia,in 1979 (ARSEF 324; Memoirs of the Entomological Society of Canada 171,287-300, 1997; e. g. Green Guard® SC from BASF Agricultural SpecialtiesPty Ltd, Australia), Metschnikowia fructicola 277 isolated from thesurface of grape berries (cv. Superior) grown in the central part ofIsrael (U.S. Pat. No. 6,994,849; NRRL Y-30752; e. g. Shemer® fromAgrogreen, Israel, now distributed by Bayer CropSciences, Germany),Microdochium dimerum L13 (CNCM I-3141; e. g. Antibot® from Agrauxine,France), Microsphaeropsis ochracea P130A isolated from apple leaves froman abandoned orchard, St-Joseph-du-Lac, Quebec, Canada in 1993 (ATCC74412; Mycologia 94(2), 297-301, 2002), Muscodor albus QST 20799 alsocalled 620 originally isolated from the bark of a cinnamon tree inHonduras (NRRL 30547; e. g. Muscudor™ or QRD300 from AgraQuest, USA),Muscodor albus SA-13 (NRRL B-50774; US 2014/0086879 A1; e. g. MBI-601-EPfrom Marrone BioInnovations, Inc., USA), Neem oil (e. g. Trilogy®,Triact® 70 EC from Certis LLC, USA), Nomuraea rileyi strains SM6101,GU87401, SR86151, CG128 and VA9101 (Braz. Arch. Biol. Technol. 46(1),13-19, 2003; WO 2013/110594), Paecilomyces lilacinus 251 isolated frominfected nematode eggs in the Philippines (AGAL 89/030550; WO1991/02051;Crop Protection 27, 352-361, 2008; e. g. BioAct®/MeloCon® from Prophyta,Germany), P. lilacinus DSM 15169 (e. g. Nemata® SC from Live SystemsTechnology S.A., Colombia), P. lilacinus BCP2 (NRRL 50756; Actaagriculturae Slovenica, 101-2, 263-275, 2013; e. g. PL Gold from BASFAgricultural Specialities (Pty) Ltd., South Africa), Paenibacillus alveiNAS6G6 (WO 2014/029697; NRRL B-50755; e.g. BAC-UP from BASF AgriculturalSpecialities (Pty) Ltd., South Africa in mixture with Bacillus pumilusKFP9F), P. polymyxa PKB1 (ATCC 202127; Can. J. Microbiol. 48(2),159-169, 2002), Pantoea agglomerans E325 (NRRL B-21856; Phytopathol.101(10), 1234-41, 2011; Trees 26, 227-238, 2012; Bloomtime Biological™from Northwest Agricultural Products, Inc., USA), Pantoea vagans(formerly agglomerans) C9-1 originally isolated in 1994 from apple stemtissue for control of fire blight in apple (J. Bacteriol. 192(24),6486-6487, 2010; e. g. BlightBan C9-1® from NuFrams America Inc., USA),Pasteuria sp. ATCC PTA-9643 (WO 2010/085795), Pasteuria sp. Ph3 isolatedfrom turfgrass soil samples collected at the DeBary Golf Course incentral Florida (ATCC SD-5832; WO 2012/064527; for control ofHoplolaimus galeatus nematode from Pasteuria Bioscience, Inc. nowSyngenta Crop Protection, LLC, USA), Pasteuria sp. Pr3 isolated fromsoil samples collected in the south-eastern United States (ATCC SD-5834;for control of Rotylenchulus reniformis nematode potentially of speciesP. ramosa; Naviva® ST from Syngenta Crop Protection, LLC, USA), P.nishizawae (WO 2010/80619), P. nishizawae Pn1 (Federal Register 76(22),5808, Feb. 2, 2011; ATCC SD-5833; e.g. Clariva™ PN from Syngenta CropProtection, LLC, USA), P. penetrans (U.S. Pat. No. 5,248,500; Del MonteCorp.), P. ramosa (WO 2010/080619), P. thornea (WO 2010/080619), P.usgae BL1 (ATCC SD-5835; J. Nematol. 42(2): 87-90, 2010; ibid. 43(2),101-109, 2011; e. g. Econem™ for control of Belonolaimus longicaudatusfrom Pasteuria BioScience now Syngenta sold by Harell's LLC, Florida,USA for use on turf for management of Belonolaimus longicaudatus),Penicillium bilaiae (also called P. bilaii) strains ATCC 18309 (=ATCC74319), ATCC 20851 and/or ATCC 22348 (=ATCC 74318) originally isolatedfrom soil in southern Alberta (Fertilizer Res. 39, 97-103, 1994; Can. J.Plant Sci. 78(1), 91-102, 1998; U.S. Pat. No. 5,026,417, WO 1995/017806;e. g. Jump Start®, Provide® from Novozymes Biologicals BioAg Group,Canada), P. bilaiae NRRL 50162 and NRRL 50169 (WO 2010/037228),Phlebiopsis gigantea (e. g. RotStop® from Verdera Oy, Finland), Pichiaanomala WRL-076 (NRRL Y-30842; U.S. Pat. No. 8,206,972), potassiumbicarbonate (e. g. Amicarb® from Stähler SA, Switzerland), potassiumsilicate (e. g. Sil-MATRIX™ from Certis LLC, USA), Pseudozyma flocculosaPF-A22 UL (e. g. Sporodex® L from Plant Products Co. Ltd., Canada),Pseudomonas sp. Proradix (DSM 13134; WO 2001/40441, e. g. PRORADIX fromSourcon Padena GmbH & Co. KG, Hechinger Str. 262, 72072 Tübingen,Germany), P. chloraphis MA 342 (Microbiology Monographs 18, 21-43, 2011;e. g. Cerall® or Cedemon® from BioAgri AB, Uppsala, Sweden or IntrachemBio Deutschland GmbH & Co. KG, Bad Camberg, Germany), P. fluorescens(e.g. in Bio Cure-B from T. Stanes & Company Limited, India; or inBlight-End from Agri Naturals, Mumbai, India), P. fluorescens A506(Phytopathol 97(2), 244-249, 2007; ATCC 31948; e. g. BlightBan® fromNuFarm Americas, Inc., Morrisville, N.C., USA), P. fluorescens ATCC13525 of biovar I=biotype A; originally isolated from pre-filter tanksin England (DSM 50090; registered for use in Canada), P. fluorescensCHA0 (Mol. Plant Microbe Interact. 5(1), 4-13, 1992), P. fluorescens CL145A (J. Invertebr. Pathol. 113(1), 104-14, 2013; e. g. Zequanox® fromMarrone BioInnovations, Davis, Calif., USA), P. fluorescens NCIB 12089(EP 0210734 A!; Victus® from Mauri Laboratories, 9 Moorebank Ave.,Moorebank, NSW 2170, Australia), P. fluorescens Pf-5 isolated from rootsurface of cotton (ATCC BAA-477), P. putida ATCC 202153 (EMBRAPA 63/88 4B; WO 2004/0245865), Pythium oligandrum DV 74 (US 2013/0035230; ATCC38472; e. g. Poyversum® from Remeslo SSRO, Biopreparaty, Czech Rep. andfrom Gowan, USA), Reynoutria sachalinensis extract (EP 0307510 B1; e. g.Regalia® SC from Marrone BioInnovations, Davis, Calif., USA or Milsana®from BioFa AG, Germany), Rhizobium leguminosarum bv. phaseoli (e. g.RHIZO-STICK from BASF Corp., USA), R. leguminosarum bv. phaseoli RG-B10(USDA 9041; from Int. J. Syst. Bacteriol. 46(1), 240-244, 1996; Int. J.Syst. Evol. Microbiol. 50, 159-170, 2000; e. g. Nodulator® Dry Bean inAfrica, HiStick NT Dry bean in US, and Nodulator® Dry Bean in Canadafrom BASF Corp., USA, or BASF Agricultural Specialties Ltd., Canada), R.I. bv. trifolii CB782 (Nodulaid® peat for Kenya white clover from BASFAgricultural Specialties Pty Ltd, Australia), R. I. bv. trifolii CC275e(Nodulaid® peat for NZ white clover from BASF Agricultural SpecialtiesPty Ltd, Australia), R. I. bv. trifolii CC283b (ICMP 4073b; Proc. NewZealand Grassland Assoc. 56, 101-105, 1994; Microbiol. 153, 3184-3195,2007; Nodulaid® peat for Caucasian clover from BASF AgriculturalSpecialties Pty Ltd, Australia), R. I. bv. trifolii CC1099 (InoculatingLegumes: A Practical Guide, ed. Grain Research and DevelopmentCorporation, 2012, ISBN 978-1-921779-45-9; e. g. Nodulaid® peat forsainfoin from BASF Agricultural Specialties Pty Ltd, Australia), R. I.bv. trifolii RP113-7 (Appl. Environ. Microbiol. 44(5), 1096-1101, 1982;e. g. Dormal® from BASF Corp., USA), R. I. bv. trifolii TA1 (Appl.Environ. Microbiol. 49(1), 127-131, 1985; e. g. Nodulaid® peat for whiteclover from BASF Agricultural Specialties Pty Ltd, Australia), R. I. bv.trifolii strain WSM1325 isolated in 1993 from the Greek Island ofSerifos (Stand. Genomic Sci. 2(3), 347-356, 2010; Inoculating Legumes: APractical Guide, ed. Grain Research and Development Corporation, 2012,ISBN 978-1-921779-45-9; Nodulaid® peat for sub clover and Nodulator®granules for sub clover both from BASF Agricultural Specialties Pty Ltd,Australia, for a broad range of annual clovers of Mediterranean origin),R. I. bv. trifolii strain WSM2304 isolated from Trifolium polymorphum inUruguay in 1998 (Stand. Genomic Sci. 2(1), 66-76, 2010), R. I. bv.viciae P1NP3Cst being a Streptomycin-resistant mutant of P1NP3C isolatedfrom pea root nodules in Bretenière, France (also referred to as 1435;New Phytol. 176, 680-690, 2007; ibid. 179(1), 224-235, 2008; e. g.Nodulator® PL Peat Granule from BASF Corp., USA; or Nodulator® XL PLfrom BASF Agricultural Specialties Ltd., Canada), R. I. bv. viciae RG-P2also called P2 isolated from pea root nodules in Sakatchewan, Canada (e.g RhizUP peat for peas and lentils in Canada from BASF AgriculturalSpecialties Ltd., Canada), R. I. bv. viciae SU303 (e. g. Nodulaid® GroupE from BASF Agricultural Specialties Pty Ltd, Australia), R. I. bv.viciae WSM1455 (e. g. Nodulaid® Group F from BASF AgriculturalSpecialties Pty Ltd, Australia), R. tropici CC511 (Agronomy, N.Z. 36,4-35, 2006; e. g. Nodulaid® peat for common bean from BASF AgriculturalSpecialties Pty Ltd, Australia), R. tropici CIAT 899 isolated inColombia (SEMIA 4077; Rev. Ciênc. Agron. 44(4) FortalezaOctober/December 2013; e. g. Nitrafix® FEIJÃO peat for beans from BASFAgricultural Specialties Ltd., Brazil in mixture with strain SEMIA4080), R. tropici H12 isolated in Planaltina, D F, Cerrados, Brazil(SEMIA 4088; Appl. Microbiol. Biotechnol. 93(5), 2035-49, 2012; e. g.Nitrafix® FEIJÃO from BASF Agricultural Specialties Ltd., Brazil), R.tropici PRF 81 isolated in Paraná, Brazil (SEMIA 4080; Soil Biology &Biochemistry 39, 867-876, 2007; BMC Microbiol. 12, 84, 2012; Nitrafix®FEIJÃO peat for beans from BASF Agricultural Specialties Ltd., Brazil inmixture with strain SEMIA 4077), Sinorhizobium meliloti RCR2011 alsocalled 2011 or SU47 (MSDJ0848; Mol. Gen. Genomics 272, 1-17, 2004; e. g.Dormal® Alfalfa & Luzerne from BASF Corp., USA; Nitragin® Gold fromNovozymes Biologicals BioAg Group, Canada), Sphaerodes mycoparasiticaSMCD2220 also called SMCD2220-01 (I DAC 301008-01; WO 2011/022809),Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) (e.g. in LITTOVIRfrom Adermatt Biocontrol, Switzerland), Steinernema carpocapsae (e. g.Millenium® from BASF Agricultural Specialities Limited, UK), S. feltiae(Nemashield® from BioWorks, Inc., USA; Nemasys® from BASF AgriculturalSpecialities Limited, UK), S. kraussei L137 (Nemasys® L from BASFAgricultural Specialities Limited, UK), Streptomyces galbus AQ6047 (NRRL30232; WO 2012/135763; AgraQuest now Bayer CropScience LP, USA); S.galbus M1064 (NRRL 50334; WO 2012/135763; AgraQuest now BayerCropScience LP, USA); S. griseoviridis K61 (Crop Protection 25, 468-475,2006; e. g. Mycostop® from Verdera Oy, Espoo, Finland), S. lydicus WYEC108 (U.S. Pat. No. 5,403,584; e. g. Actinovate® from Natural Industries,Inc., USA), S. violaceusniger YCED-9 (U.S. Pat. No. 5,968,503; e. g.DT-9® from Natural Industries, Inc., USA), Talaromyces flavus V117bisolated from soil (e. g. Protus® WG from Prophyta, Germany), andUlocladium oudemansii HRU3 (Agronomy 3, 632-647, 2013; e. g. Botry-Zen®from Botry-Zen Ltd, NZ).

Strains can be obtained from culture collections and deposition centers(listed by their acronym=strain prefix here:http://www.wfcc.info/ccinfo/collection/by_acronym/) such as strains withprefices AGAL or NMI from: National Measurement Institute, 1/153 BertieStreet, Port Melbourne, Victoria, Australia 3207; ATCC: American TypeCulture Collection, 10801 University Blvd., Manassas, Va. 20110-2209,USA; BR: Embrapa Agrobiology Diazothrophic Microbial Culture Collection,P.O.Box 74.505, Seropedica, Rio de Janeiro, 23.851-970, Brazil; CABI orIMI: CABI Europe—International Mycological Institute, Bakeham Lane,Egham, Surrey, TW20 9TYNRRL, UK; CB: The CB Rhizobium Collection, Schoolof Environment and Agriculture, University of Western Sydney,Hawkesbury, Locked Bag 1797, South Penrith Distribution Centre, NSW1797, Australia; CBS: Centraalbureau voor Schimmelcultures, FungalBiodiversity Centre, Uppsalaan 8, PO Box 85167, 3508 AD Utrecht,Netherlands; CC: Division of Plant Industry, CSIRO, Canberra, Australia;CNCM: Collection Nationale de Cultures de Microorganismes, InstitutePasteur, 25 rue du Docteur Roux, F-75724 PARIS Cedex 15; CPAC:Embrapa-Cerrados, CX. Postal 08223, Planaltina, DF, 73301-970, Brazil;DSM: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen andZellkulturen GmbH, Inhoffenstraβe 7 B, 38124 Braunschweig, Germany;IDAC: International Depositary Authority of Canada Collection, Canada;ICMP: Interntional Collection of Micro-organisms from Plants, LandcareResearch, Private Bag 92170, Auckland Mail Centre, Auckland 1142, NewZealand; IITA: IITA, PMB 5320, Ibadan, Nigeria; INTA: AgricultureCollection Laboratory of the Instituto de Microbiologia y ZoologiaAgricola (IMYZA), Instituto Nacional de Tecnologi'a Agropecuaria (INTA),Castelar, Argentina; MSDJ: Laboratoire de Microbiologie des Sols, INRA,Dijon, France; MUCL: Mycothèque de l'Université catholique de Louvain,Croix du Sud 2, box L7.05.06, 1348 Louvain-la-Neuve, Belgium; NCIMB orNICB: The National Collections of Industrial and Marine Bacteria Ltd.,Torry Research Station, P.O. Box 31, 135 Abbey Road, Aberdeen, AB9 8DG,Scotland; Nitragin: Nitragin strain collection, The Nitragin Company,Milwaukee, Wis., USA, NRRL or ARSEF (collection of entomopathogenicfungi): ARS Culture Collection of the National Center for AgriculturalUtilization Research, Agricultural Research Service, U.S. Department ofAgriculture, 1815 North University Street, Peoria, Ill. 61604, USA; NZP:Department of Scientific and Industrial Research Culture Collection,Applied Biochemistry Division, Palmerston North, New Zealand; PPRI:ARC-Plant Protection Research Institute, Private Bag X134, QueenswoodPretoria, Gauteng, 0121, South Africa; SEMIA: FEPAGRO-Fundação Estadualde Pesquisa Agropecuária, Rua Gonçalves Dias, 570, Bairro Menino Deus,Porto Alegre/RS, Brazil; SRDI: SARDI, Adelaide, South Australia; USDA:U.S. Department of Agriculture, Agricultural Research Service, Soybeanand Alfalfa Research Laboratory, BARC-West, 10300 Baltimore Boulevard,Building 011, Beltsville, Md. 20705, USA (Beltsville Rhiz. Cult.Catalog: http://pdf.usaid.gov/pdf_docs/PNAAW891.pdf); and WSM: MurdochUniversity, Perth, Western Australia. Further strains may be found at:http://gcm.wfcc.info/; http://www.landcareresearch.co.nz/resources.collections/icmp.

Jasmonic acid, its salts (jasmonates) or derivatives include withoutlimitation potassium, sodium, lithium, ammonium, dimethylammonium,isopropylammonium, diolammonium and diethtriethanolammonium jasmonate;and also jasmonic acid methyl ester, jasmonic acid amide, jasmonic acidmethylamide, jasmonic acid-L-amino acid (amide-linked) conjugates (e. g.conjugates with L-isoleucine, L-valine, L-leucine, or L-phenylalanine),12-oxo-phytodienoic acid, coronatine, coronalon, coronafacoyl-L-serine,coronafacoyl-L-threonine, methyl esters of 1-oxo-indanoyl-isoleucine,methyl esters of 1-oxo-indanoyl-leucine, cis-jasmone, linoleic acid orderivatives thereof, and combinations of any of the above.

Humates are humic and fulvic acids extracted from a form of lignite coaland clay, known as leonardite. Humic acids are organic acids that occurin humus and other organically derived materials such as peat andcertain soft coal. They have been shown to increase fertilizerefficiency in phosphate and micro-nutrient uptake by plants as well asaiding in the development of plant root systems.

According to one embodiment of the inventive mixtures, the at least onepesticide II is selected from the groups L1) to L6):

-   -   L1) Microbial pesticides with fungicidal, bactericidal,        viricidal and/or plant defense activator activity selected from:        Ampelomyces quisqualis M-10 (L.1.1), Aspergillus flavus NRRL        21882 (L1.2), Aureobasidium pullulans DSM 14940 (L1.3), A.        pullulans DSM 14941 (L.1.4), Bacillus altitudinis 41KF2b        (L.1.5), B. amyloliquefaciens IN937a (L.1.11), B.        amyloliquefaciens IT-45 (L.1.12), B. amyloliquefaciens ssp.        plantarum D747 (L.1.13), B. amyloliquefaciens ssp. plantarum        FZB24 (L.1.14), B. amyloliquefaciens ssp. plantarum FZB42        (L.1.15), B. amyloliquefaciens ssp. plantarum GB03 (L.1.16), B.        amyloliquefaciens ssp. MBI600 (NRRL B-50595) (L.1.17), B.        amyloliquefaciens ssp. plantarum QST-713 (L.1.18), B. mycoides        AQ726 (L.1.21), B. mycoides strain J (L.1.22), B. pumilus INR-7        (L.1.23), B. pumilus KFP9F (L.1.24), B. pumilus QST 2808        (L.1.25), B. pumilus GHA 180 (L.1.26), B. simplex ABU 288        (L.1.27), B. subtilis CX-9060 (L.1.29), B. subtilis FB17        (L.1.30), B. subtilis GB07 (L.1.31), Candida oleophila I-82        (L.1.32), C. oleophila O (L.1.33), C. saitoana (L.1.34),        Clavibacter michiganensis (bacteriophages) (L.1.35),        Coniothyrium minitans CON/M/91-08 (L.1.36), Cryphonectria        parasitica (L.1.37), Cryptococcus albidus (L.1.38),        Dilophosphora alopecuri (L.1.39), Fusarium oxysporum (L.1.40),        Clonostachys rosea f. catenulata J1446 (L.1.41), Gliocladium        roseum 321U (L.1.42), Metschnikowia fructicola NRRL Y-30752        (L.1.43), Microdochium dimerum (L.1.44), Microsphaeropsis        ochracea P130A (L.1.45), Muscodor albus QST 20799 (L.1.46),        Muscodor albus SA-13 (L.1.47), Paenibacillus alvei NAS6G6        (L.1.48), Paenibacillus polymyxa PKB1 (L.1.49), Pantoea        agglomerans E325 (L.1.90), Pantoea vagans C9-1 (L.1.50),        Penicillium bilaiaeATCC 22348 (L.1.51), P. bilaiae ATCC 20851        (L.1.52), Penicillium bilaiae ATCC 18309 (L.1.53), Phlebiopsis        gigantea (L.1.54), Pichia anomala WRL-76 (L.1.55), Pseudomonas        sp. Proradix (L.1.56), Pseudomonas chloraphis MA 342        (L.1.57), P. fluorescens A506 (L.1.58), P. fluorescens CL 145A        (L.1.91), P. fluorescens NCIB 12089 (L.1.92), P. fluorescens        Pf-5 (L.1.93), P. fluorescens WCS 374 (L.1.94), P. fluorescens        ATCC 13525 (L.1.95), P. fluorescens CHA0 (L.1.96), P. putida        ATCC 202153 (L.1.97), Pseudozyma flocculosa PF-A22 UL (L.1.59),        Pythium oligandrum DV 74 (L.1.60), Sphaerodes mycoparasitica        SMCD2220 (L.1.61), Streptomyces griseoviridis K61 (L.1.62), S.        lydicus WYEC 108 (L.1.63), S. violaceusniger XL-2 (L.1.64), S.        violaceusniger YCED-9 (L.1.65), Talaromyces flavus V117b        (L.1.66), Typhula phacorrhiza 94671 (L.1.86), Ulocladium        oudemansii H RU3 (L.1.87), Verticillium dahlia (L.1.88),        zucchini yellow mosaic virus (avirulent strain) (L.1.89);    -   L2) Biochemical pesticides with fungicidal, bactericidal,        viricidal and/or plant defense activator activity selected from:        chitosan (hydrolysate) (L.2.1), harpin protein (L.2.2),        laminarin (L.2.3), Menhaden fish oil (L.2.4), natamycin (L.2.5),        Plum pox virus coat protein (L.2.6), potassium bicarbonate        (L.2.7), Reynoutria sachalinensis extract (L.2.8), salicylic        acid (L.2.9), potassium or sodium bicarbonate (L.2.10), tea tree        oil (L.2.11);    -   L3) Microbial pesticides with insecticidal, acaricidal,        molluscidal and/or nematicidal activity selected from:        Agrobacterium radiobacter K1026 (L.3.1), A. radiobacter K84        (L.3.2), Bacillus firmus I-1582 (L.3.3); B. thuringiensis ssp.        aizawai strains: ABTS-1857 (L.3.4), SAN 401 I (L.3.5), ABG-6305        (L.3.6) and ABG-6346 (L.3.7); B. t. ssp. israelensis AM65-52        (L.3.8), B. t. ssp. israelensis SUM-6218 (L.3.9), B. t. ssp.        galleriae SDS-502 (L.3.10), B. t. ssp. kurstaki EG 2348        (L.3.11), B. t. ssp. kurstaki SB4 (L.3.12), B. t. ssp. kurstaki        ABTS-351 (HD-1) (L.3.13), Beauveria bassiana ATCC 74040        (L.3.14), B. bassiana GHA (L.3.15), B. bassiana H123        (L.3.16), B. bassiana DSM 12256 (L.3.17), B. bassiana PPRI 5339        (L.3.18), B. brongniartii (L.3.19), Burkholderia sp. A396        (L.3.20), Chromobacterium subtsugae PRAA4-1 (L.3.21), Cydia        pomonella granulosis virus V22 (L.3.22), Cydia pomonella        granulosis virus V1 (L.3.23), Cryptophlebia leucotreta        granulovirus (CrleGV) (L.3.57), Flavobacterium sp. H492        (L.3.60), Helicoverpa armigera nucleopolyhedrovirus (HearNPV)        (L.3.58), Isaria fumosorosea Apopka-97 (L.3.24), Lecanialium        longisporum KV42 (L.3.25), L. longisporum KV71 (L.3.26), L.        muscarium KV01 (L.3.27), Metarhizium anisopliae FI-985        (L.3.28), M. anisopliae FI-1045 (L.3.29), M. anisopliae F52        (L.3.30), M. anisopliae ICIPE 69 (L.3.31), M. anisopliae var.        acridum IMI 330189 (L.3.32); Nomuraea rileyi strains: SA86101        (L.3.33), GU87401 (L.3.34), SR86151 (L.3.35), CG128 (L.3.36) and        VA9101 (L.3.37); Paecilomyces fumosoroseus FE 9901 (L.3.38), P.        lilacinus 251 (L.3.39), P. lilacinus DSM 15169 (L.3.40), P.        lilacinus BCP2 (L.3.41), Paembacillus popilliae Dutky-1940 (NRRL        B-2309=ATCC 14706) (L.3.42), P. popilliae Dutky 1 (L.3.43), P.        popilliae KLN 3 (L.3.56), Pasteuria sp. Ph3 (L.3.44), Pasteuria        sp. ATCC PTA-9643 (L.3.45), Pasteuria sp. ATCC SD-5832        (L.3.46), P. nishizawae Pn1 (L.3.46), P. penetrans (L.3.47), P.        ramosa (L.3.48), P. sp. Pr-3 (L.3.49), P. thornea (L.3.50), P.        usgae (L.3.51), Pseudomonas fluorescens CL 145A (L.3.52),        Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) (L.3.59),        Steinernema carpocapsae (L.3.53), S. feltiae (L.3.54), S.        kraussei L137 (L.3.55);    -   L4) Biochemical pesticides with insecticidal, acaricidal,        molluscidal, pheromone and/or nematicidal activity selected        from: L-carvone (L.4.1), citral (L.4.2),        (E,Z)-7,9-dodecadien-1-yl acetate (L.4.3), ethyl formate        (L.4.4), (E,Z)-2,4-ethyl decadienoate (pear ester) (L.4.5),        (Z,Z,E)-7,11,13-hexadecatrienal (L.4.6), heptyl butyrate        (L.4.7), isopropyl myristate (L.4.8), cis-jasmone (L.4.9),        lavanulyl senecioate (L.4.10), 2-methyl 1-butanol (L.4.11),        methyl eugenol (L.4.12), methyl jasmonate (L.4.13),        (E,Z)-2,13-octadecadien-1-ol (L.4.14),        (E,Z)-2,13-octadecadien-1-ol acetate (L.4.15),        (E,Z)-3,13-octadecadien-1-ol (L.4.16), R-1-octen-3-ol (L.4.17),        pentatermanone (L.4.18), potassium silicate (L.4.19), sorbitol        actanoate (L.4.20), (E,Z,Z)-3,8,11-tetradecatrienyl acetate        (L.4.21), (Z,E)-9,12-tetradecadien-1-yl acetate (L.4.22),        Z-7-tetradecen-2-one (L.4.23), Z-9-tetradecen-1-yl acetate        (L.4.24), Z-11-tetradecenal (L.4.25), Z-11-tetradecen-1-ol        (L.4.26), Acacia negra extract (L.4.27), extract of grapefruit        seeds and pulp (L.4.28), extract of Chenopodium ambrosiodes        (L.4.29), Catnip oil (L.4.30), Neem oil (L.4.31), Quillay        extract (L.4.32), Tagetes oil (L.4.33);    -   L5) Microbial pesticides with plant stress reducing, plant        growth regulator, plant growth promoting and/or yield enhancing        activity selected from: Azospirillum amazonense BR 11140 (SpY2)        (L.5.1), A. brasilense Ab-V5 (L.5.74), A. brasilense Ab-V6        (L.5.75), A. brasilense AZ39 (L.5.2), A. brasilense XOH        (L.5.3), A. brasilense Sp245 (BR 11005) (L.5.4), A. brasilense        BR 11002 (L.5.5), A. lipoferum BR 11646 (Sp31) (L.5.6), A.        irakense (L.5.7), A. halopraeferens (L.5.8), Bradyrhizobium sp.        PNL01 (L.5.9), B. sp. (Arachis) CB1015 (L.5.10), B. sp.        (Arachis) USDA 3446 (L.5.11), B. sp. (Arachis) SEMIA 6144        (L.5.12), B. sp. (Arachis) SEMIA 6462 (L.5.13), B. sp. (Arachis)        SEMIA 6464 (L.5.14), B. sp. (Vigna) (L.5.15), B. elkanii SEMIA        587 (L.5.16), B. elkanii SEMIA 5019 (L.5.17), B. elkanii U-1301        (L.5.18), B. elkanii U-1302 (L.5.19), B. elkanii USDA 74        (L.5.20), B. elkanii USDA 76 (L.5.21), B. elkanii USDA 94        (L.5.22), B. elkanii USDA 3254 (L.5.23), B. japonicum 532c        (L.5.24), B. japonicum CPAC 15 (L.5.25), B. japonicum E-109        (L.5.26), B. japonicum G49 (L.5.27), B. japonicum TA-11        (L.5.28), B. japonicum USDA 3 (L.5.29), B. japonicum USDA 31        (L.5.30), B. japonicum USDA 76 (L.5.31), B. japonicum USDA 110        (L.5.32), B. japonicum USDA 121 (L.5.33), B. japonicum USDA 123        (L.5.34), B. japonicum USDA 136 (L.5.35), B. japonicum SEMIA 566        (L.5.36), B. japonicum SEMIA 5079 (L.5.37), B. japonicum SEMIA        5080 (L.5.38), B. japonicum WB74 (L.5.39), B. liaoningense        (L.5.40), B. lupini LL13 (L.5.41), B. lupini WU425 (L.5.42), B.        lupini WSM471 (L.5.43), B. lupini WSM4024 (L.5.44), Glomus        intraradices RTI-801 (L.5.45), Mesorhizobium sp. WSM1271        (L.5.46), M. sp. WSM1497 (L.5.47), M. ciceri CC1192 (L.5.48), M.        huakii (L.5.49), M. loti CC829 (L.5.50), M. loti SU343 (L.5.51),        Rhizobium leguminosarum bv. phaseoli RG-B10 (L.5.52), R. I. bv.        trifolii RP113-7 (L.5.53), R. I. bv. trifolii 095 (L.5.57), R.        I. bv. trifolii TA1 (L.5.58), R. I. bv. trifolii CC283b        (L.5.59), R. I. bv. trifolii CC275e (L.5.60), R. I. bv. trifolii        CB782(L.5.61), R. I. bv. trifolii CC1099 (L.5.62), R. I. bv.        trifolii WSM1325 (L.5.63), R. I. bv. viciae SU303 (L.5.64), R.        I. bv. viciae WSM1455 (L.5.65), R. I. bv. viciae P1NP3Cst        (L.5.66), R. I. bv. viciae RG-P2 (L.5.67), R. tropici PRF 81        (L.5.68), R. tropici SEMIA 4077 (L.5.69), R. tropici        CC511(L.5.70), Sinorhizobium meliloti RCR2011 (L.5.71), S.        meliloti NRG185 (L.5.72), S. meliloti RRI128 (L.5.73);    -   L6) Biochemical pesticides with plant stress reducing, plant        growth regulator and/or plant yield enhancing activity selected        from: abscisic acid (L.6.1), aluminium silicate (kaolin)        (L.6.2), 3-decen-2-one (L.6.3), formononectin (L.6.4), genistein        (L.6.5), hesperetin (L.6.6), homobrassinolide (L.6.7), humates        (L.6.8), methyl jasmonate (L.6.9), cis-jasmone (L.6.10),        lysophosphatidyl ethanlamine (L.6.11), naringenin (L.6.12),        polymeric polyhydroxy acid (L.6.13), salicylic acid (L.6.14),        Ascophyllum nodosum (Norwegian kelp, Brown kelp) extract        (L.6.15) and Ecklonia maxima (kelp) extract (L.6.16).

The present invention furthermore relates to agrochemical compositionscomprising a mixture of at least one microorganism I (component 1) andat least one biopesticide selected from the group L) (component 2), inparticular at least one further fungicidal biopesticide selected fromthe groups L1) and L2), as described above, and if desired at least onesuitable auxiliary.

Preference is also given to mixtures comprising as pesticide II(component 2) a biopesticide from group L1), preferably selected fromBacillus amyloliquefaciens herein even more preferably from strainsIN937a, IT-45; B. amyloliquefaciens ssp. plantarum (formerly called B.subtilis or B. subtilis spp. amyloliquefaciens) herein even morepreferably from strains MBI600, D747, FZB254, FZB42, GB03, and QST-713;B. pumilus herein even more preferably from strains GHA 180, INR-7,KFP9F and QST 2808; B. simplex herein more preferably strain ABU 288; B.solisalsi; B. subtilis herein even more preferably selected from strainsCX-9060, FB17 and GB07; Muscodor albus herein more preferably strainsQST 20799 and SA-13; Paenibacillus alvei herein more preferably strainNAS6G6, Paenibacillus polymyxa herein more preferably strain PKB1,Penicillium bilaiae herein more preferably strains ATCC 22348, ATCC20581 and ATCC 18309; Pseudomonas fluorescens herein more preferablystrain A506; Sphaerodes mycoparasitica herein more preferably strainSMCD2220.

Preference is also given to mixtures comprising as pesticide II(component 2) a biopesticide from group L1), even more preferablyselected from even more preferably from B. amyloliquefaciens ssp.plantarum M B1600, B. amyloliquefaciens ssp. plantarum QST-713, B.pumilus INR-7, B. pumilus QST 2808, B. simplex ABU 288, B. subtilisFB17, and Paenibacillus alvei NAS6G6.

According to one embodiment of the inventive mixtures, the at least onepesticide II is Bacillus amyloliquefaciens ssp. plantarum MBI600. Thesemixtures are particularly suitable in soybean.

According to another embodiment of the inventive mixtures, the at leastone pesticide II is B. pumilus INR-7. These mixtures are particularlysuitable in soybean and corn.

According to a further embodiment, the at least one pesticide II isBacillus simplex, preferably B. simplex ABU 288. These mixtures areparticularly suitable in soybean and corn.

According to a further embodiment, the at least one pesticide II isBacillus subtilis, preferably B. subtilis strain FB17.

According to one embodiment of the inventive mixtures, the at least onepesticide II is selected from Bacillus amyloliquefaciens spp. plantarumFZB24, B. amyloliquefaciens ssp. plantarum FZB42, B. amyloliquefaciensssp. plantarum D747, B. amyloliquefaciens ssp. plantarum MBI600, B.amyloliquefaciens spp. plantarum GB03, B. amyloliquefaciens spp.plantarum QST-713, B. pumilus GB34, B. pumilus INR-7, B. pumilus KFP9F,B. pumilus QST 2808, B. pumilus GHA 180, B. simplex ABU 288, B. subtilisCX-9060, B. subtilis FB17 and B. subtilis GB07. These mixtures areparticularly suitable in soybean and corn, in particular for seedtreatment.

According to one embodiment of the inventive mixtures, the at least onepesticide II is Coniothyrium minitans CON/M/91-08. These mixtures areparticularly suitable for seed and/or soil treatment.

According to a further embodiment, the at least one pesticide II isselected from Pseudomonas spp., preferably selected from P. chloraphisherein more preferably strain MA 342 and Pseudomonas sp. DSM 13134; P.fluorescens herein more preferably selected from strains A506, WCS 374and Pf-5; and P. putida herein more preferably strain ATCC 202153.

The present invention also relates to mixtures wherein the at least onepesticide II is selected from the fungal species Muscodor albuspreferably from the strains SA-13 and QST 20799, which are particularlysuiable for soil and seed treatment against soil-borne pathogens and/ornematodes.

Preference is also given to mixtures comprising as pesticide II(component 2) a biopesticide from group L2), preferably selected fromchitosan (hydrolysate), methyl-jasmonate, cis-jasmone, laminarin,Reynoutria sachalinensis extract and tea tree oil; even more preferablefrom methyl jasmonate and cis-jasmone.

Preference is also given to mixtures comprising as pesticide II(component 2) a biopesticide from group L3), preferably selected fromAgrobacterium radiobacter herein preferably strain K1026, Bacillusfirmus herein referably strain I-1582, Bacillus thuringiensis ssp.kurstaki herein preferably strain SB4, Beauveria bassiana hereinpreferably selected from strains GHA, H123, DSM 12256 and PPRI 5339;Burkholderia sp. and herein preferably strain A396, Metarhiziumanisopliae var. acridum herein preferably strain IMI 330189, M.anisopliae herein preferably selected from strains FI-985, FI-1045, F52and ICIPE 69; Paecilomyces lilacinus herein preferably selected fromstrains 251, DSM 15169 and BCP2, Paenibacillus popilliae hereinpreferably selected from strains Dutky-1940, KLN 3 and Dutky 1;Pasteuria nishazawa and herein preferably strain Pn1.

Preference is also given to mixtures comprising as pesticide II(component 2) a biopesticide from group L3), even more preferably fromBacillus thuringiensis ssp. kurstaki SB4, B. bassiana DSM 12256, B.bassiana PPRI 5339, Paecilomyces lilacinus DSM 15169, P. lilacinus BCP2,P. lilacinus 251, Paenibacillus popilliae Dutky-1940, P. popilliae KLN 3and P. popilliae Dutky 1.

According to a further embodiment, the at least one pesticide II isBeauveria bassiana PPRI 5339.

According to a further embodiment, the at least one pesticide II isBacillus firmus, preferably spores of strain CNCM I-1582, preferablyuseful for seed treatment of soybean and corn against nematodes andinsects.

According to a further embodiment, the at least one pesticide II isBacillus cereus, preferably spores of CNCM I-1562, preferably useful forseed treatment of soybean and corn against nematodes and insects.

According to a further embodiment, the at least one pesticide II is amixture of spores of B. firmus and B. cereus, preferably mixtures sporesof above mentioned strains CNCM I-1582 and CNCM I-1562, preferablyuseful for seed treatment of soybean and corn against nematodes andinsects.

According to a further embodiment, the at least one pesticide II isselected from Bacillus t. ssp. kurstaki preferably from strains EG 2348,SB4 and ABTS-351 (HD-1), in particular B. t. ssp. kurstaki SB4. Thesestrains are used for control of lepidopteran larvae, but withoutnoctuidae.

According to one embodiment of the inventive mixtures, the at least onepesticide II is selected from Bacillus firmus CNCM I-1582, Paecilomyceslilcinus 251, Pasteuria nishizawa Pn1 and Burkholderia sp. A396 havingnematicidal, acaricidal and/or insecticidal activity. These mixtures areparticularly suitable in soybean and corn, in particular for seedtreatment.

Preference is also given to mixtures comprising as pesticide II(component 2) a biopesticide from group L4), preferably selected frommethyl jasmonate, Acacia negra extract, extract of grapefruit seeds andpulp, Catnip oil, Neem oil, Quillay extract and Tagetes oil, inparticular methyl jasmonate or water-based Quillay extract.

Preference is also given to mixtures comprising as pesticide II(component 2) a biopesticide from group L5), preferably selected fromAzospialum amazonense, A. brasllense, A. lipoferum, A. irakense, A.halopraeferens, Bradyrhizobium sp. (Vigna), B. elkanil; B. japonicumPaembacillus alvei, Penicilium bilaiae, Rhizobium leguminosarum bv.phaseoli; R. I. bv. trifolii, R. I. bv. viciae, and Sinorhizobiummeliloti.

Preference is also given to mixtures comprising as pesticide II(component 2) a biopesticide from group L5) selectedifrom Azospialumamazonense SpY2^(T) , A. brasllense XOH, A. brasilense Sp245, A.brasilense Cd, A. brasllense Ab-V5, A. brasilense Ab-V6, A. lipoferumSp31, Bradyrhizobium sp. (Vigna) PNL1, B. elkanii SEMIA 587, B. elkaniiSEMIA 5019, B. japonicum SEMIA 5079, B. japonicum SEMIA 5080, B.japonicum TA-11, B. japonicum 532c, Paembacillus alvei NAS6G6,Peniciillium bilaiae strains ATCC 18309, ATCC 20851 and ATCC 22348;Rhizobium leguminosarum bv. phaseoli P1NP3Cst, R. I. bv. phaseoliRG-B10, R. I. bv. trifolii RP113-7, R. I. bv. viciae SU303, R. I. bv.viciae WSM1455, R. tropici SEMIA 4077, R. tropici PRF 81 andSinorhizobium meliloti, even more preferably selected from Azospirillumbrasilense Sp245, Bradyrhizobium sp. (Vigna) PNL1, B. elkanii SEMIA 587,B. elkanii SEMIA 5019, B. japonicum SEMIA 5079, B. japonicum SEMIA 5080,B. japonicum TA-11 and B. japonicum 532c.

According to a further embodiment, component 2) in the mixturescomprises at least one biopesticide II strain selected from Azospirillumbrasilense Sp 245, A. brasilense Ab-V5, A. brasilense Ab-V6,Bradyrhizobium elkanii SEMIA 5019, B. elkanii SEMIA 587, B. japonicum532c, B. japonicum E-109, B. japonicum SEMIA 5079 and B. japonicum SEMIA5080.

The present invention also relates to mixtures, wherein the at least onepesticide II is selected from B. japonicum and B. elkanii and furthercomprises a pesticide III, wherein pesticide III is selected fromjasmonic acid, its salts and derivatives thereof, preferablymethyl-jasmonate or cis-jasmone.

The present invention also relates to mixtures wherein the at least onepesticide II is selected from Bacillus amyloliquefaciens ssp. plantarumM B1600, B. amyloliquefaciens ssp. plantarum FZB24, B. amyloliquefaciensssp. plantarum FZB42, B. amyloliquefaciens ssp. plantarum D747, B.amyloliquefaciens ssp. plantarum QST-713, B. firmus CNCM I-1582, B.pumilus GHA 180, B. pumilus INR-7, B. pumilus QST 2808, B. simplex ABU288, B. subtilis FB17, Burkholderia sp. A396, Coniothyrium minitansCON/M/91-08, Paecllomyces lilacinus 251, Paenibacillus alvei NAS6G6,Pasteuria nishizawae Pn1, P. bilaiae ATCC 18309, P. bilaiae ATCC 20851,P. bilaiae ATCC 22348; cis-jasmone, methyl jasmonate and harpin protein

The pesticides II of chemical nature (chemical pesticides) are describedby common names, their preparation and their activity against pests isknown (cf.: http://www.alanwood.net/pesticides/); these pesticides areoften commercially available.

Preference is also given to mixtures comprising as component 2) at leastone pesticide II from the groups A) to H), N) and O) selected from:

-   -   A) Inhibitors of complex III at Q_(o) site selected from:        pyraclostrobin, azoxystrobin, picoxystrobin, trifloxystrobin,        dimoxystrobin, enestroburin, fenaminstrobin, fluoxastrobin,        kresoxim-methyl, mandestrobine, metominostrobin, orysastrobin,        pyrametostrobin, pyraoxystrobin;        -   inhibitors of complex II selected from: fluxapyroxad,            boscalid, benzovindiflupyr, penflufen, penthiopyrad,            sedaxane, fluopyram, bixafen, flutolanil, isofetamid,            isopyrazam; carboxin, benodanil, fenfuram, flutolanil,            furametpyr, mepronil, oxycarboxin, thifluzamide; other            respiration inhibitor: silthiofam;    -   B) DMI fungicides selected from: ipconazole, difenoconazole,        prothioconazole, prochloraz, triticonazole, flutriafol,        cyproconazole, diniconazole, diniconazole-M, fluquinconazole,        flusilazole, hexaconazole, imazalil, imibenconazole,        metconazole, myclobutanil, simeconazole, tebuconazole,        triadimenol, uniconazole;    -   C) Nucleic acid synthesis inhibitors selected from: metalaxyl,        mefenoxam;    -   D) Inhibitors of cell division and cytoskeleton selected from:        thiabendazole, thiophanate-methyl, carbendazim; ethaboxam;    -   F) MAP/histidine kinase inhibitor: fludioxonil;    -   G) Lipid and membrane synthesis inhibitors selected from:        dimethomorph, zoxamide, flumorph, mandipropamid, pyrimorph,        benthiavalicarb, iprovalicarb, oxathiapiprolin, valifenalate;    -   H) Inhibitors with Multi Site Action selected from: thiram,        ziram;    -   N) Herbicides selected from: glyphosate and dicamba;    -   O) Insecticides selected from:        -   organo(thio)phosphates selected from: acephate,            chlorpyrifos,        -   carbamates selected from: methiocarb, thiodicarb;        -   pyrethroids selected from: tefluthrin, bifenthrin,            cypermethrin, alpha-cypermethrin, cyfluthrin,            beta-cyfluthrin, lambda-cyhalothrin, deltamethrin,            esfenvalerate, etofenprox, fenvalerate, flucythrinate,            permethrin;        -   macrocyclic lactone insecticides selected from: abamectin,            spinosad;        -   nicotinic receptor agonists/antagonists compounds selected            from: clothianidin, imidacloprid, thiamethoxam, dinotefuran,            acetamiprid, flupyradifurone, thiacloprid, triflumezopyrim,            nitenpyram, sulfoxaflor;        -   GABA antagonist compounds selected from: fipronil,            ethiprole, vaniliprole, pyrafluprole, pyriprole,            5-amino-1-(2,6-dichloro-4-methyl-phenyl)-4-sulfinamoyl-1H-pyrazole-3-carbothioic            acid amide;        -   ryanodine receptor inhibitors selected from:            chlorantraniliprole, cyantraniliprole, and flubendiamide;            more preferably said mixtures comprise as component 1) B.            amyloliquefaciens AP-188.

Preference is also given to mixtures comprising as component 2) at leastone pesticide II from pyraclostrobin, azoxystrobin, trifloxystobin;fluxapyroxad, penflufen, sedaxane, fluopyram; ipconazole;oxathiapiprolin, valifenalate; fipronil; imidacloprid;chlorantraniliprole; and cyantraniliprole.

Particular preference is also given to mixtures comprising ascomponent 1) B. amyloliquefaciens AP-188 and as component 2) at leastone pesticide II from pyraclostrobin, azoxystrobin, trifloxystobin;fluxapyroxad, penflufen, sedaxane, fluopyram; ipconazole;oxathiapiprolin, valifenalate; fipronil; imidacloprid;chlorantraniliprole; and cyantraniliprole.

The inventive mixtures comprising at least one microorganism I and/or aspesticide II a microbial pesticide from groups L1), L3) and L5) may beformulated as an inoculant for a plant. The term “inoculant” means apreparation that includes an isolated culture of a microbial pesticideand optionally a carrier, which may include a biologically acceptablemedium.

Herein, microbial pesticides may be supplied in any physiological statesuch as active or dormant. Dormant microbial pesticides may be suppliedfor example frozen, dried, or lyophilized or partly desiccated(procedures to produce partly desiccated organisms are given in WO2008/002371) or in form of spores.

Microbial pesticides II selected from groups L1), L3) and L5) and the atleast one microorganism I used as organisms in an active state can bedelivered in a growth medium without any additional additives ormaterials or in combination with suitable nutrient mixtures.

The at least one microorganism I is preferably delivered and formulatedin a dormant stage, more preferably in form of spores.

The mixtures and compositions according to the invention are suitable asfungicides. They are distinguished by an outstanding effectivenessagainst a broad spectrum of phytopathogenic fungi, including soil-bornefungi, which derive especially from the classes of thePlasmodiophoromycetes, Peronosporomycetes (syn. Oomycetes),Chytridiomycetes, Zygomycetes, Ascomycetes, Basidiomycetes andDeuteromycetes (syn. Fungi imperfecti). Some are systemically effectiveand they can be used in crop protection as foliar fungicides, fungicidesfor seed dressing and soil fungicides. Moreover, they are suitable forcontrolling harmful fungi, which inter alia occur in wood or roots ofplants.

The mixtures and compositions according to the invention areparticularly important in the control of a multitude of phytopathogenicfungi on various cultivated plants; and on the plant propagationmaterial, such as seeds, and the crop material of these plants.

Preferably the inventive mixtures and compositions are used forcontrolling a multitude of fungi on the following cultivated plants:field crops, such as potatoes sugar beets, tobacco, wheat, rye, barley,oats, rice, corn, cotton, soybeans, rape, legumes, sunflowers, coffee orsugar cane; fruits; vines; ornamentals; or vegetables, such ascucumbers, tomatoes, beans or squashes.

The term “plant propagation material” is to be understood to denote allthe generative parts of the plant such as seeds and vegetative plantmaterial such as cuttings and tubers (e. g. potatoes), which can be usedfor the multiplication of the plant. This includes seeds, roots, fruits,tubers, bulbs, rhizomes, shoots, sprouts and other parts of plants,including seedlings and young plants, which are to be transplanted aftergermination or after emergence from soil.

Preferably, treatment of plant propagation materials with the inventivemixtures and compositions thereof, respectively, is used for controllinga multitude of fungi on cereals, such as wheat, rye, barley and oats;rice, corn, cotton and soybeans.

The term “cultivated plants” is to be understood as including plantswhich have been modified by breeding, mutagenesis or genetic engineeringincluding but not limiting to agricultural biotech products on themarket or in development (cf. http://cera-gmc.org/, see GM crop databasetherein). Genetically modified plants are plants, which genetic materialhas been so modified by the use of recombinant DNA techniques that undernatural circumstances cannot readily be obtained by cross breeding,mutations or natural recombination. Typically, one or more genes havebeen integrated into the genetic material of a genetically modifiedplant in order to improve certain properties of the plant.

The inventive mixtures and compositions are particularly suitable forcontrolling the following plant diseases:

Albugo spp. (white rust) on ornamentals, vegetables (e. g. A. candida)and sunflowers (e. g. A. tragopogonis); Altemana spp. (Alternaria leafspot) on vegetables, rape (A. brassicola or A. brassicae), sugar beets(A. tenuis), fruits, rice, soybeans, potatoes (e. g. A. solani or A.altemata), tomatoes (e. g. A. solani or A. altemata) and wheat;Aphanomyces spp. on sugar beets and vegetables; Ascochyta spp. oncereals and vegetables, e. g. A. tritici (anthracnose) on wheat and A.hordei on barley; Bipolaris and Drechslera spp. (teleomorph:Cochliobolus spp.) on corn (e. g. D. maydis), cereals (e. g. B.sorokiniana: spot blotch), rice (e. g. B. oryzae) and turfs; Blumeria(formerly Erysiphe) graminis (powdery mildew) on cereals (e. g. on wheator barley); Botrytis cinerea (teleomorph: Botrifotinia fuckeliana: greymold) on fruits and berries (e. g. strawberries), vegetables (e. g.lettuce, carrots, celery and cabbages), rape, flowers, vines, forestryplants and wheat; Bremia lactucae (downy mildew) on lettuce;Ceratocystis (syn. Ophiostoma) spp. (rot or wilt) on broad-leaved treesand evergreens, e. g. C. ulmi (Dutch elm disease) on elms; Cercosporaspp. (Cercospora leaf spots) on corn, rice, sugar beets (e. g. C.beticola), sugar cane, vegetables, coffee, soybeans (e. g. C. sojina orC. kikuchii) and rice; Cladosporium spp. on tomatoes (e. g. C. fulvum:leaf mold) and cereals, e. g. C. herbarum (black ear) on wheat;Claviceps purpurea (ergot) on cereals; Cochliobolus (anamorph:Helminthosporium of Bipolaris) spp. (leaf spots) on corn (C. carbonum),cereals (e. g. C. sativus, anamorph: B. sorokiniana) and rice (e. g. C.miyabeanus, anamorph: H. oryzae); Colletotrichum (teleomorph:Glomerella) spp. (anthracnose) on cotton (e. g. C. gossypii), corn (e.g. C. graminicola), soft fruits, potatoes (e. g. C. coccodes black dot),beans (e. g. C. lindemuthianum) and soybeans (e. g. C. truncatum or C.gloeosporioides); Corticium spp., e. g. C. sasakii (sheath blight) onrice; Corynespora cassiicola (leaf spots) on soybeans and ornamentals;Cycloconium spp., e. g. C. oleaginum on olive trees; Cylindrocarpon spp.(e. g. fruit tree canker or young vine decline, teleomorph: Nectria orNeonectria spp.) on fruit trees, vines (e. g. C. liriodendri,teleomorph: Neonectria liriodendri: Black Foot Disease) and ornamentals;Dematophora (teleomorph: Rosellinia) necatrix (root and stem rot) onsoybeans; Diaporthe spp., e. g. D. phaseolorum (damping off) onsoybeans; Drechslera (syn. Helminthosporium, teleomorph: Pyrenophora)spp. on corn, cereals, such as barley (e. g. D. teres, net blotch) andwheat (e. g. D tritici-repentis: tan spot), rice and turf; Esca(dieback, apoplexy) on vines, caused by Formitipona (syn. Phellinus)punctata, F. mediterranea, Phaeomoniella chlamydospora (earlierPhaeoacremonium chlamydosporum), Phaeoacremonium aleophllum and/orBotryosphaeria obtusa; Elsinoe spp. on pome fruits (E. pyri), softfruits (E. veneta: anthracnose) and vines (E. ampelina: anthracnose);Entyloma oryzae (leaf smut) on rice; Epicoccum spp. (black mold) onwheat; Erysiphe spp. (powdery mildew) on sugar beets (E. betae),vegetables (e. g. E. pisi), such as cucurbits (e. g. E. cichoracearum),cabbages, rape (e. g. E. cruciferarum); Eutypa lata (Eutypa canker ordieback, anamorph: Cytosporina lata, syn. Libertella blepharis) on fruittrees, vines and ornamental woods; Exserohilum (syn. Helminthosporium)spp. on corn (e. g. E. turcicum); Fusarium (teleomorph: Gibberella) spp.(wilt, root or stem rot) on various plants, such as F. graminearum or F.culmorum (root rot, scab or head blight) on cereals (e. g. wheat orbarley), F. oxysporum on tomatoes, F. solani on soybeans and F.verticillioides on corn; Gaeumannomyces graminis (take-all) on cereals(e. g. wheat or barley) and corn; Gibberella spp. on cereals (e. g. G.zeae) and rice (e. g. G. fujikuroi: Bakanae disease); Glomerellacingulata on vines, pome fruits and other plants and G. gossypii oncotton; Grainstaining complex on rice; Guignardia bidwellii (black rot)on vines; Gymnosporangium spp. on rosaceous plants and junipers, e. g.G. sabinae (rust) on pears; Helminthosporium spp. (syn. Drechslera,teleomorph: Cochliobolus) on corn, cereals and rice; Hemileia spp., e.g. H. vastatrix (coffee leaf rust) on coffee; Isariopsis clavispora(syn. Cladosporium vitis) on vines; Macrophomina phaseolina (syn.phaseoli) (root and stem rot) on soybeans and cotton; Microdochium (syn.Fusarium) nivale (pink snow mold) on cereals (e. g. wheat or barley);Microsphaera diffusa (powdery mildew) on soybeans; Monilinia spp., e. g.M. laxa, M. fructicola and M. fructigena (bloom and twig blight, brownrot) on stone fruits and other rosaceous plants; Mycosphaerella spp. oncereals, bananas, soft fruits and ground nuts, such as e. g. M.graminicola (anamorph: Septoria tritici, Septoria blotch) on wheat;Peronospora spp. (downy mildew) on cabbage (e. g. P. brassicae), rape(e. g. P. parasitica), onions (e. g. P. destructor), tobacco (P.tabacina) and soybeans (e. g. P. manshurica); Phakopsora pachyrhizi andP. meibomiae (soybean rust) on soybeans; Phialophora spp. e. g. on vines(e. g. P. tracheiphila and P. tetraspora) and soybeans (e. g. P.gregata: stem rot); Phoma lingam (root and stem rot) on rape and cabbageand P. betae (root rot, leaf spot and damping-off) on sugar beets;Phomopsis spp. on sunflowers, vines (e. g. P. viticola: can and leafspot) and soybeans (e. g. stem rot: P. phaseoli, teleomorph: Diaporthephaseolorum); Physoderma maydis (brown spots) on corn; Phytophthora spp.(wilt, root, leaf, fruit and stem root) on various plants, such aspaprika and cucurbits (e. g. P. capsici), soybeans (e. g. P. megasperma,syn. P. sojae), potatoes and tomatoes (e. g. P. infestans: late blight)and broad-leaved trees (e. g. P. ramorum: sudden oak death);Plasmodiophora brassicae (club root) on cabbage, rape, radish and otherplants; Plasmopara spp., e. g. P. viticola (grapevine downy mildew) onvines and P. halstedii on sunflowers; Podosphaera spp. (powdery mildew)on rosaceous plants, hop, pome and soft fruits, e. g. P. leucotricha onapples; Polymyxa spp., e. g. on cereals, such as barley and wheat (P.graminis) and sugar beets (P. betae) and thereby transmitted viraldiseases; Pseudocercosporella herpotrichoides (eyespot, teleomorph:Tapesia yallundae) on cereals, e. g. wheat or barley; Pseudoperonospora(downy mildew) on various plants, e. g. P. cubensis on cucurbits or P.humili on hop; Pseudopezicula tracheiphila (red fire disease or‘rotbrenner’, anamorph: Phialophora) on vines; Puccinia spp. (rusts) onvarious plants, e. g. P. triticina (brown or leaf rust), P. striiformis(stripe or yellow rust), P. hordei (dwarf rust), P. graminis (stem orblack rust) or P. recondita (brown or leaf rust) on cereals, such as e.g. wheat, barley or rye, and asparagus (e. g. P. asparagi); Pyrenophora(anamorph: Drechslera) tritici-repentis (tan spot) on wheat or P. teres(net blotch) on barley; Pyricularia spp., e. g. P. oryzae (teleomorph:Magnaporthe grisea, rice blast) on rice and P. grisea on turf andcereals; Pythium spp. (damping-off) on turf, rice, corn, wheat, cotton,rape, sunflowers, soybeans, sugar beets, vegetables and various otherplants (e. g. P. ultimum or P. aphani-dermatum); Ramularia spp., e. g.R. collo-cygni (Ramularia leaf spots, Physiological leaf spots) onbarley and R. beticola on sugar beets; Rhizoctonia spp. on cotton, rice,potatoes, turf, corn, rape, potatoes, sugar beets, vegetables andvarious other plants, e. g. R. solani (root and stem rot) on soybeans,R. solani (sheath blight) on rice or R. cerealis (Rhizoctonia springblight) on wheat or barley; Rhizopus stolonifer (black mold, soft rot)on strawberries, carrots, cabbage, vines and tomatoes; Rhynchosporiumsecalis (scald) on barley, rye and triticale; Sarocladium oryzae and S.attenuatum (sheath rot) on rice; Sclerotinia spp. (stem rot or whitemold) on vegetables and field crops, such as rape, sunflowers (e. g. S.sclerotiorum) and soybeans (e. g. S. rolfsii or S. sclerotiorum);Septoria spp. on various plants, e. g. S. glycines (brown spot) onsoybeans, S. tritici (Septoria blotch) on wheat and S. (syn.Stagonospora) nodorum (Stagonospora blotch) on cereals; Uncinula (syn.Erysiphe) necator (powdery mildew, anamorph: Oidium tuckeri) on vines;Setospaeria spp. (leaf blight) on corn (e. g. S. turcicum, syn.Helminthosporium turcicum) and turf; Sphacelotheca spp. (smut) on corn,(e. g. S. reiliana: head smut), sorghum and sugar cane; Sphaerothecafuliginea (powdery mildew) on cucurbits; Spongospora subterranea(powdery scab) on potatoes and thereby transmitted viral diseases;Stagonospora spp. on cereals, e. g. S. nodorum (Stagonospora blotch,teleomorph: Leptosphaeria [syn. Phaeosphaeria] nodorum) on wheat;Synchytrium endobioticum on potatoes (potato wart disease); Taphrinaspp., e. g. T. deformans (leaf curl disease) on peaches and T. pruni(plum pocket) on plums; Thielaviopsis spp. (black root rot) on tobacco,pome fruits, vegetables, soybeans and cotton, e. g. T. basicola (syn.Chalara elegans); Tilletia spp. (common bunt or stinking smut) oncereals, such as e. g. T. tritici (syn. T. caries, wheat bunt) and T.controversa (dwarf bunt) on wheat; Typhula incamata (grey snow mold) onbarley or wheat; Urocystis spp., e. g. U. occulta (stem smut) on rye;Uromyces spp. (rust) on vegetables, such as beans (e. g. U.appendiculatus, syn. U. phaseoli) and sugar beets (e. g. U. betae);Ustilago spp. (loose smut) on cereals (e. g. U. nuda and U. avaenae),corn (e. g. U. maydis: corn smut) and sugar cane; Venturia spp. (scab)on apples (e. g. V. inaequalis) and pears; and Verticillium spp. (wilt)on various plants, such as fruits and ornamentals, vines, soft fruits,vegetables and field crops, e. g. V. dahliae on strawberries, rape,potatoes and tomatoes.

The mixtures and compositions according to the invention are alsosuitable as bactericides. They are distinguished by an outstandingeffectiveness against a broad spectrum of phytopathogenic bacteria,including soil-borne bacteria, which derive especially from the generaof Agrobacterium, Clavibacter, Corynebacterium, Erwinia, Leifsonia,Pectobacterium, Pseudomonas, Ralstonia, Xanthomonas (e.g. Xanthomonasoryzae causing bacterial blight on rice) and Xylella; preferablyErwinia; even more preferably Erwinia amylovora causing fire blight onapples, pears and other member of the family Rosaceae.

The mixtures according to the present invention and compositionsthereof, respectively, are also suitable for controlling harmful fungiin the protection of stored products or harvest.

The mixtures and compositions according to the invention areparticularly important in the control of a multitude of phytopathogenicinsects or other pests (e.g. lepidopterans, beetles, dipterans, thrips,heteropterans, hemiptera, homoptera, termites, orthopterans, arachnids,and nematodes) on various cultivated plants.

Preferably the inventive mixtures and compositions are used forcontrolling a multitude of pests on field crops, such as potatoes sugarbeets, tobacco, wheat, rye, barley, oats, rice, corn, cotton, soybeans,rape, legumes, sunflowers, coffee or sugar cane; fruits; vines;ornamentals; or vegetables, such as cucumbers, tomatoes, beans orsquashes.

The inventive mixtures and the compositions thereof, respectively, areparticularly suitable for controlling the following harmful insects fromthe order of the

lepidopterans (Lepidoptera), for example Agrotis ypsilon, Agrotissegetum, Alabama argillacea, Anticarsia gemmatalis, Argyresthiaconjugella, Autographa gamma, Bupalus piniarius, Cacoecia murinana,Capua reticulana, Cheimatobia brumata, Choristoneura fumiferana,Choristoneura occidentalis, Cirphis unipuncta, Cydia pomonella,Dendrolimus pini, Diaphania nitidalis, Diatraea grandiosella, Eariasinsulana, Elasmopalpus lignosellus, Eupoecilia ambiguella, Evetriabouliana, Feltia subterranea, Galleria mellonella, Grapholithafunebrana, Grapholitha molesta, Heliothis armigera, Heliothis virescens,Heliothis zea, Hellula undalis, Hibernia defoliaria, Hyphantria cunea,Hyponomeuta malinellus, Keiferia lycopersicella, Lambdina fiscellaria,Laphygma exigua, Leucoptera coffeella, Leucoptera scitella,Lithocolletis blancardella, Lobesia botrana, Loxostege sticticalis,Lymantria dispar, Lymantria monacha, Lyonetia clerkella, Malacosomaneustria, Mamestra brassicae, Orgyia pseudotsugata, Ostrinia nubilalis,Panoils flammea, Pectinophora gossypiella, Peridroma saucia, Phalerabucephala, Phthorimaea operculella, Phyllocnistis citrella, Pierisbrassicae, Plathypena scabra, Plutella xylostella, Pseudoplusiaincludens, Rhyacionia frustrana, Scrobipalpula absoluta, Sitotrogacerealella, Sparganothis pilleriana, Spodoptera frugiperda, Spodopteralittoralis, Spodoptera litura, Thaumatopoea pityocampa, Tortrixviridana, Trichoplusia ni and Zeiraphera canadensis,

beetles (Coleoptera), for example Agrilus sinuatus, Agriotes lineatus,Agriotes obscurus, Amphimallus solstitialis, Anisandrus dispar,Anthonomus grandis, Anthonomus pomorum, Atomaria linearis, Blastophaguspiniperda, Blitophaga undata, Bruchus rufimanus, Bruchus pisorum,Bruchus lentis, Byctiscus betulae, Cassida nebulosa, Cerotomatrifurcata, Ceuthorrhynchus assimilis, Ceuthorrhynchus napi, Chaetocnematibialis, Conoderus vespertinus, Crioceris asparagi, Diabroticalongicornis, Diabrotica speciosa, Diabrotica 12-punctata, Diabroticavirgifera, Diloboderus abderus, Epilachna varivestis, Epitrixhirtipennis, Eutinobothrus brasiliensis, Hylobius abietis, Hyperabrunneipennis, Hypera postica, Ips typographus, Lema bilineata, Lemamelanopus, Leptinotarsa decemlineata, Limonius californicus,Lissorhoptrus olyzophilus, Melanotus communis, Meligethes aeneus,Melolontha hippocastani, Melolontha melolontha, Oulema oryzae,Ortiorrhynchus sulcatus, Oryazophagus oryzae, Otiorrhynchus ovatus,Phaedon cochleariae, Phyllotreta chrysocephala, Phyllophaga sp.,Phyllophaga cuyabana, Phyllophaga triticophaga, Phyllopertha horticola,Phyllotreta nemorum, Phyllotreta striolata, Popillia japonica, Sitonalineatus and Sitophilus granaria,

dipterans (Diptera), for example Aedes aegypti, Aedes vexans, Anastrephaludens, Anopheles maculipennis, Ceratitis capitata, Chrysomya bezziana,Chrysomya hominivorax, Chrysomya macellaria, Contarinia sorghicola,Cordylobia anthropophaga, Culex pipiens, Dacus cucurbitae, Dacus oleae,Dasineura brassicae, Fannia canicularis, Gasterophilus intestinalis,Glossina morsitans, Haematobia irritans, HaplodiplosIS equestris,Hylemyia platura, Hypoderma lineata, Liriomyza sativae, Liriomyzatrifolil, Lucilia caprin, Lucilia cuprina, Lucilia sericata, Lycoriapectoralis, Mayetiola destructor, Musca domestica, Muscina stabulans,Oestrus ovis, Oscinella frit, Pegomya hysocyami, Phorbia antiqua,Phorbia brassicae, Phorbia coarctata, Rhagoletis cerasi, Rhagoletispomonella, Tabanus bovinus, Tipula oleracea and Tipula paludosa,

thrips (Thysanoptera), e.g. Frankliniella fusca, Frankliniellaoccidentalis, Frankliniella tritici, Scirtothrips citri, Thrips oryzae,Thrips palmi and Thrips tabaci,

hymenopterans (Hymenoptera), e.g. Acromyrmex ambuguus, Acromyrmexcrassispinus, Acromyrmex heiery, Acromyrmex landolti, Acromyrmexsubterraneus, Athalia rosae, Atta capiguara, Atta cephalotes, Attalaevigata, Atta robusta, Atta sexdens, Atta texana, Hoplocampa minuta,Hoplocampa testudinea, Monomorium pharaonic, Solenopsis geminata andSolenopsis invicta,

heteropterans (Heteroptera), e.g. Acrosternum Mare, Blissus leucopterus,Cyrtopeltis notatus, Dichelops furcatus, Dysdercus cingulatus, Dysdercusintermedius, Euchistos heros, Eurygaster integriceps, Euschistusimpictiventris, Leptoglossus phyllopus, Lygus lineolaris, Lyguspratensis, Nezara viridula, Piesma quadrata, Piezodorus gulldim, Solubeainsularis and Thyanta perditor,

Hemiptera and Homoptera, e.g. Acrosternum Mare, Blissus leucopterus,Cyrtopeltis notatus, Diaphorina citri, Dysdercus cingulatus, Dysdercusintermedius, Eurygaster integriceps, Euschistus impictiventris,Leptoglossus phyllopus, Lygus lineolaris, Lygus pratensis, Nezaraviridula, Piesma quadrata, Solubea insularis, Thyanta perditor,Acyrthosiphon onobrychis, Adelges laricis, Aphidula nasturtii, Aphisfabae, Aphis forbesi, Aphis pomi, Aphis gossypii, Aphis grossulariae,Aphis schneideri, Aphis spiraecola, Aphis sambuci, Acyrthosiphon pisum,Aulacorthum solani, Brachycaudus cardui, Brachycaudus helichrysi,Brachycaudus persicae, Brachycaudus prunicola, Brevicoryne brassicae,Capitophorus horni, Cerosipha gossypii, Chaetosiphon fragaefolii,Cryptomyzus ribis, Dreyfusia nordmannianae, Dreyfusia piceae, Dysaphisradicola, Dysaulacorthum pseudosolani, Dysaphic plantaginea, Dysaphispyri, Empoasca fabae, Hyalopterus pruni, Hyperomyzus lactucae,Macrosiphum avenae, Macrosiphum euphorbiae, Macrosiphon rosae, Megouraviciae, Melanaphis pyrarius, Metopolophium dirhodum, Myzodes persicae,Myzus ascalonicus, Myzus cerasi, Myzus varians, Nasonovia ribis-nigri,Nilaparvata lugens, Pemphigus bursarius, Perkinsiella saccharicida,Phorodon humuli, Psylla mali, Psylla piri, Rhopalomyzus ascalonicus,Rhopalosiphum maidis, Rhopalosiphum padi, Rhopalosiphum insertum,Sappaphis mala, Sappaphis mali, Schizaphis graminum, Schizoneuralanuginosa, Sitobion avenae, Trialeurodes vaporariorum, Toxopteraaurantiiand, Viteus vitifolii, Cimex lectularius, Cimex hemipterus,Reduvius senilis, Triatoma spp., and Arilus critatus,

termites (Isoptera), e.g. Calotermes flavicollis, Cornitermes cumulans,Heterotermes tenuis, Leucotermes flavipes, Neocapritemes opacus,Procornitermes triacifer; Reticulitermes lucifugus, Syntermes molestus,and Termes natalensis,

orthopterans (Orthoptera), e.g. Acheta domestica, Blatta orientalis,Blattella germanica, Forticula auricularia, Gryllotalpa gryllotalpa,Locusta migratoria, Melanoplus bivittatus, Melanoplus femur-rubrum,Melanoplus mexicanus, Melanoplus sanguinipes, Melanoplus spretus,Nomadacris septemfasciata, Periplaneta americana, Schistocercaamericana, Schistocerca peregrina, Stauronotus maroccanus and Tachycinesasynamorus,

Arachnoidea, such as arachnids, e.g. of the families Argasidae, Ixodidaeand Sarcoptidae, such as Amblyomma americanum, Amblyomma variegatum,Argas persicus, Boophilus annulatus, Boophilus decoloratus, Boophilusmicroplus, Dermacentor silvarum, Hyalomma truncatum, Ixodes ricinus,Ixodes rubicundus, Ornithodorus moubata, Otobius megnini, Dermanyssusgallinae, Psoroptes ovis, Rhipicephalus appendiculatus, Rhipicephalusevertsi, Sarcoptes scabiei, and Eriophyidae spp. such as Aculusschlechtendali, Phyllocoptrata oleivora and Eriophyes sheldoni;Tarsonemidae spp. such as Phytonemus pallidus and Polyphagotarsonemuslatus; Tenuipalpidae spp. such as Brevipalpus phoenicis; Tetranychidaespp. such as Tetranychus cinnabarinus, Tetranychus kanzawai, Tetranychuspacificus, Tetranychus telarius and Tetranychus urticae, Panonychusulmi, Panonychus citri, and Oligonychus pratensis.

In particular, the inventive mixtures are suitable for combating pestsof the orders Coleoptera, Lepidoptera, Thysanoptera, Homoptera,Isoptera, and Orthoptera.

They are also suitable for controlling the following plant parasiticnematodes such as root-knot nematodes, Meloidogyne arenaria, Meloidogynechitwoodi, Meloidogyne exigua, Meloidogyne hapla, Meloidogyne incognita,Meloidogyne javanica and other Meloidogyne species; cyst nematodes,Globodera rostochiensis, Globodera pallida, Globodera tabacum and otherGlobodera species, Heterodera avenae, Heterodera glycines, Heteroderaschachtii, Heterodera trifolii and other Heterodera species; seed gallnematodes, Anguina funesta, Anguina tritici and other Anguina species;stem and foliar nematodes, Aphelenchoides besseyi, Aphelenchoidesfragariae, Aphelenchoides ritzemabosi and other Aphelenchoides species;sting nematodes, Belonolaimus longicaudatus and other Belonolaimusspecies; pine nematodes, Bursaphelenchus xylophilus and otherBursaphelenchus species; ring nematodes, Criconema species, Criconemellaspecies, Criconemoides species, and Mesocriconema species; stem and bulbnematodes, Ditylenchus destructor, Ditylenchus dipsaci, Ditylenchusmyceliophagus and other Ditylenchus species; awl nematodes, Dolichodorusspecies; spiral nematodes, Helicotylenchus dihystera, Helicotylenchusmulticinctus and other Helicotylenchus species, Rotylenchus robustus andother Rotylenchus species; sheath nematodes, Hemicycliophora species andHemicriconemoides species; Hirshmanniella species; lance nematodes,Hoplolaimus columbus, Hoplolaimus galeatus and other Hoplolaimusspecies; false root-knot nematodes, Nacobbus aberrans and other Nacobbusspecies; needle nematodes, Longidorus elongates and other Longidorusspecies; pin nematodes, Paratylenchus species; lesion nematodes,Pratylenchus brachyurus, Pratylenchus coffeae, Pratylenchus curvitatus,Pratylenchus goodeyi, Pratylencus neglectus, Pratylenchus penetrans,Pratylenchus scribneri, Pratylenchus vulnus, Pratylenchus zeae and otherPratylenchus species; Radinaphelenchus cocophilus and otherRadinaphelenchus species, burrowing nematodes, Radopholus similis andother Radopholus species; reniform nematodes, Rotylenchulus reniformisand other Rotylenchulus species; Scutellonema species; stubby rootnematodes, Trichodorus primitivus and other Trichodorus species;Paratrichodorus minor and other Paratrichodorus species, stuntnematodes, Tylenchorhynchus claytoni, Tylenchorhynchus dubius and otherTylenchorhynchus species and Merlinius species; citrus nematodes,Tylenchulus semipenetrans and other Tylenchulus species; daggernematodes, Xiphinema americanum, Xiphinema index, Xiphinemadiversicaudatum and other Xiphinema species; and other plant parasiticnematode species

Plant propagation materials may be treated with the mixtures andcompositions of the invention prophylactically either at or beforeplanting or transplanting.

In particular, the present invention relates to a method for protectionof plant propagation material from pests, wherein the plant propagationmaterial is treated with an effective amount of an inventive mixture.

In a preferred embodiment, the present invention relates to a method forprotection of plant propagation material from animal pests (insects,acarids or nematodes), wherein the plant propagation material aretreated with an effective amount of an inventive mixture.

In an equally preferred embodiment, the present invention relates to amethod for protection of plant propagation material from harmful fungi,wherein the plant propagation material is treated with an effectiveamount of an inventive mixture.

For example, for seed treatment and soil applications, it is evidentthat a plant suffering from fungal or insecticidal attack shows reducedgermination and emergence leading to poorer plant or crop establishmentand vigor, and consequently, to a reduced yield as compared to a plantpropagation material which has been subjected to curative or preventivetreatment against the relevant pest and which can grow without thedamage caused by the biotic stress factor. However, the methodsaccording to the invention lead to an enhanced plant health even in theabsence of any biotic stress. This means that the positive effects ofthe mixtures of the invention cannot be explained just by the pesticidalactivities of microorganisms I and pesticides II, but are based onfurther activity profiles. Accordingly, the application of the inventivemixtures can also be carried out in the absence of pest pressure.

The term “plant health” is to be understood to denote a condition of theplant and/or its products which is determined by several indicatorsalone or in combination with each other such as yield (e. g. increasedbiomass and/or increased content of valuable ingredients), plant vigor(e. g. improved plant growth and/or greener leaves (“greening effect”)),quality (e. g. improved content or composition of certain ingredients)and tolerance to abiotic and/or biotic stress.The above identifiedindicators for the health condition of a plant may be interdependent, ormay result from each other.

In an equally preferred embodiment, the present invention relates to amethod for improving the health of plants grown from said plantpropagation material, wherein the plant propagation material is treatedwith an effective amount of an inventive mixture.

In an equally preferred embodiment, the present invention relates to amethod for improving the health of plants, wherein the plant is treatedwith an effective amount of an inventive mixture.

Each plant health indicator such as yield, plant vigor, quality andtolerance of the plant to abiotic and/or biotic stress, is to beunderstood as a preferred embodiment of the present invention eithereach on its own.

The invention also relates to agrochemical compositions comprising anauxiliary and at least one microorganism I, or a cell-free extractthereof or at least one metabolite thereof having pesticidal activity,and/or a mutant of a microorganism I having pesticidal activity andproducing at least one pesticidal metabolite as defined herein, or apesticidal metabolite or extract of the mutant, and at least onepesticide II according to the invention.

An agrochemical composition comprises a fungicidally or insecticidallyeffective amount of at least one microorganism I and at least onepesticide II. Such an amount can vary in a broad range and is dependenton various factors, such as the fungal or pest species to be controlled,the treated cultivated plant or material, the climatic conditions.

In the case of mixtures comprising microbial pesticides II selected fromgroups L1), L3) and L5), the microorganisms as used according to theinvention can be cultivated continuously or discontinuously in the batchprocess or in the fed batch or repeated fed batch process. A review ofknown methods of cultivation will be found in the textbook by Chmiel(Bioprozesstechnik 1. Einführung in die Bioverfahrenstechnik (GustavFischer Verlag, Stuttgart, 1991)) or in the textbook by Storhas(Bioreaktoren and periphere Einrichtungen (Vieweg Verlag,Braunschweig/Wiesbaden, 1994)). The culture medium that is to be usedmust satisfy the requirements of the particular strains in anappropriate manner. Descriptions of culture media for variousmicroorganisms are given in the handbook “Manual of Methods for GeneralBacteriology” of the American Society for Bacteriology (Washington D.C., USA, 1981). Information on media optimization can be found in thetextbook “Applied Microbiol. Physiology, A Practical Approach” (Publ. P.M. Rhodes, P. F. Stanbury, IRL Press (1997) p. 53-73, ISBN 0 19 9635773).

According to one embodiment, individual components of the compositionaccording to the invention such as parts of a kit or parts of a binaryor ternary mixture may be mixed by the user himself in a spray tank orany other kind of vessel used for applications (e.g seed treater drums,seed pelleting machinery, knapsack sprayer) and further auxiliaries maybe added, if appropriate. When living microorganisms, such asmicroorganisms I and pesticides II from groups L1), L3) and L5), formpart of such kit, it must be taken care that choice and amounts of theother parts of the kit (e.g. chemcial pesticidal agents) and of thefurther auxiliaries should not influence the viability of the microbialpesticides in the composition mixed by the user. Especially forbactericides and solvents, compatibility with the respective microbialpesticide has to be taken into account.

Consequently, one embodiment of the invention is a kit for preparing ausable pesticidal composition, the kit compring a) a compositioncomprising component 1) as defined herein and at least one auxiliary;and b) a composition comprising component 2) as defined herein and atleast one auxiliary.

The at least one pesticide II can be converted into customary types ofagrochemical compositions, e. g. solutions, emulsions, suspensions,dusts, powders, pastes, granules, pressings, capsules, and mixturesthereof. Examples for composition types are suspensions (e.g. SC, OD,FS), emulsifiable concentrates (e.g. EC), emulsions (e.g. EW, EO, ES,ME), capsules (e.g. CS, ZC), pastes, pastilles, wettable powders ordusts (e.g. WP, SP, WS, DP, DS), pressings (e.g. BR, TB, DT), granules(e.g. WG, SG, GR, FG, GG, MG), insecticidal articles (e.g. LN), as wellas gel formulations for the treatment of plant propagation materialssuch as seeds (e.g. GF). These and further compositions types aredefined in the “Catalogue of pesticide formulation types andinternational coding system”, Technical Monograph No. 2, 6^(th) Ed. May2008, CropLife International.

The compositions are prepared in a known manner, such as described byMollet and Grubemann, Formulation technology, Wiley VCH, Weinheim, 2001;or Knowles, New developments in crop protection product formulation,Agrow Reports DS243, T&F Informa, London, 2005.

When living microorganisms, such as microorganisms I and pesticides IIfrom groups L1), L3) and L5), form part of the compositions, suchcompositions can be prepared as compositions comprising besides theactive ingredients at least one auxiliary (inert ingredient) by usualmeans (see e.g. H. D. Burges: Formulation of Micobial Biopestcides,Springer, 1998). Suitable customary types of such compositions aresuspensions, dusts, powders, pastes, granules, pressings, capsules, andmixtures thereof. Examples for composition types are suspensions (e.g.SC, OD, FS), capsules (e.g. CS, ZC), pastes, pastilles, wettable powdersor dusts (e.g. WP, SP, WS, DP, DS), pressings (e.g. BR, TB, DT),granules (e.g. WG, SG, GR, FG, GG, MG), insecticidal articles (e.g. LN),as well as gel formulations for the treatment of plant propagationmaterials such as seeds (e.g. GF). Herein, it has to be taken intoaccount that each formulation type or choice of auxiliary should notinfluence the viability of the microorganism during storage ofthecomposition and when finally applied to the soil, plant or plantpropagation material. Suitable formulations are e.g. mentioned in WO2008/002371, U.S. Pat. No. 6,955,912, U.S. Pat. No. 5,422,107.

Examples for suitable auxiliaries are those mentioned earlier herein,wherein it must be taken care that choice and amounts of suchauxiliaries should not influence the viability of the microbialpesticides in the composition. Especially for bactericides and solvents,compatibility with the respective microorganism of the respectivemicrobial pesticide has to be taken into account. In addition,compositions with microbial pesticides may further contain stabilizersor nutrients and UV protectants. Suitable stabilzers or nutrients aree.g. alpha-tocopherol, trehalose, glutamate, potassium sorbate, varioussugars like glucose, sucrose, lactose and maltodextrine (H. D. Burges:Formulation of Micobial Biopestcides, Springer, 1998). Suitable UVprotectants are e.g. inorganic compouns like titan dioxide, zinc oxideand iron oxide pigments or organic compounds like benzophenones,benzotriazoles and phenyltriazines. The compositions may in addition toauxiliaries mentioned for compositions comprising compounds I hereinoptionally comprise 0.1-80% stabilizers or nutrients and 0.1-10% UVprotectants.

The agrochemical compositions generally are characterized in that theycontain an effective quantity of the active components as defined above.Generally, they contain between 0.01 and 95%, preferably between 0.1 and90%, and in particular between 0.5 and 75%, by weight of activecomponents, in particular active substances.

According to one embodiment, the compositions contain microbialpesticides such as TJ1000 and pesticides II from groups L1), L3) and L5)in an amount from 1×10⁵ to 1×10¹² CFU, preferably from 1×10⁷ CFU to1×10¹² CFU, more preferably from 1×10⁹ CFU to 1×10¹² CFU per gram totalweight of the composition.

Solutions for seed treatment (LS), suspoemulsions (SE), flowableconcentrates (FS), powders for dry treatment (DS), water-dispersiblepowders for slurry treatment (WS), water-soluble powders (SS), emulsions(ES), emulsifiable concentrates (EC) and gels (GF) are usually employedfor the purposes of treatment of plant propagation materials,particularly seeds.

Preferred examples of seed treatment formulation types or soilapplication for pre-mix compositions are of WS, LS, ES, FS, WG orCS-type.

The compositions in question give, after two-to-tenfold dilution, activecomponents concentrations of from 0.01 to 60% by weight, preferably from0.1 to 40%, in the ready-to-use preparations. Application can be carriedout before or during sowing. Methods for applying or treating compound Iand compound II and compositions thereof, respectively, on to plantpropagation material, especially seeds include dressing, coating,pelleting, dusting, soaking and in-furrow application methods of thepropagation material. Preferably, compound I and compound II or thecompositions thereof, respectively, are applied on to the plantpropagation material by a method such that germination is not induced,e. g. by seed dressing, pelleting, coating and dusting.

It is preferred that the plant propagation material is a seed, seedpiece (i.e. stalk) or seed bulb.

Although it is believed that the present method can be applied to a seedin any physiological state, it is preferred that the seed be in asufficiently durable state that it incurs no damage during the treatmentprocess. Typically, the seed would be a seed that had been harvestedfrom the field; removed from the plant; and separated from any cob,stalk, outer husk, and surrounding pulp or other non-seed plantmaterial. The seed would preferably also be biologically stable to theextent that the treatment would cause no biological damage to the seed.It is believed that the treatment can be applied to the seed at any timebetween harvest of the seed and sowing of the seed or during the sowingprocess (seed directed applications). The seed may also be primed eitherbefore or after the treatment.

Even distribution of the ingredients in inventive mixtures and adherencethereof to the seeds is desired during propagation material treatment.Treatment could vary from a thin film (dressing) of the formulationcontaining the combination, for example, a mixture of activeingredient(s), on a plant propagation material, such as a seed, wherethe original size and/or shape are recognizable to an intermediary state(such as a coating) and then to a thicker film (such as pelleting withmany layers of different materials (such as carriers, for example,clays; different formulations, such as of other active ingredients;polymers; and colourants) where the original shape and/or size of theseed is no longer recognizable.

Seed can be treated by applying thereto the component 1) and component2) present in the inventive mixtures in any desired sequence orsimultaneously.

The seed treatment occurs to an unsown seed, and the term “unsown seed”is meant to include seed at any period between the harvest of the seedand the sowing of the seed in the ground for the purpose of germinationand growth of the plant. Treatment to an unsown seed is not meant toinclude those practices in which the active ingredient is applied to thesoil but would include any application practice that would target theseed during the planting process.

Preferably, the treatment occurs before sowing of the seed so that thesown seed has been pre-treated with the combination. In particular, seedcoating or seed pelleting are preferred in the treatment of thecombinations according to the invention. As a result of the treatment,the ingredients in each combination are adhered on to the seed andtherefore available for pest control.

The treated seeds can be stored, handled, sowed and tilled in the samemanner as any other active ingredient treated seed.

In particular, the present invention relates to a method for protectionof plant propagation material from pests and/or improving the health ofplants grown from said plant propagation material, wherein the soil,wherein plant propagation material is sown, is treated with an effectiveamount of an inventive mixture.

In particular, the present invention relates to a method for protectionof plant propagation material from pests, wherein the soil, whereinplant propagation material is sown, is treated with an effective amountof an inventive mixture.

In particular, the present invention relates to a method for protectionof plant propagation material from harmful fungi, wherein the soil,wherein plant propagation material is sown, is treated with an effectiveamount of an inventive mixture.

In particular, the present invention relates to a method for protectionof plant propagation material from animal pests (insects, acarids ornematodes), wherein the soil, wherein plant propagation material issown, is treated with an effective amount of an inventive mixture.

When employed in plant protection, the total amounts of activecomponents applied are, depending on the kind of effect desired, from0.001 to 10 kg per ha, preferably from 0.005 to 2 kg per ha, morepreferably from 0.05 to 0.9 kg per ha, in particular from 0.1 to 0.75 kgper ha. In the case of microorganisms I and microbial pesticides II, theapplication rates for foliar or soil (e. g. in furrow) applicationmethods preferably range from about 1×10⁶ to 5×10¹⁵ (or more) CFU/ha andeven more preferably, the spore concentration is about 1×10⁷ to about1×10¹² CFU/ha.

When employed in plant protection by seed treatment, the amount of theinventive mixtures (based on total weight of active components) is inthe range from 0.01-10 kg, preferably from 0.1-1000 g, more preferablyfrom 1-100 g per 100 kilogram of plant propagation material (preferablyseeds). In the case of microorganisms I and microbial pesticides II, theapplication rates with respect to plant propagation material preferablyrange from about 1×10⁶ to 1×10¹² (or more) CFU/seed. Preferably, theconcentration is about 1×10⁶ to about 1×10¹¹ CFU/seed. In the case ofmicroorganisms I and microbial pesticides II, the application rates withrespect to plant propagation material also preferably range from about1×10⁷ to 1×10¹⁴ (or more) CFU per 100 kg of seed, preferably from 1×10⁹to about 1×10¹¹ CFU per 100 kg of seed.

When used in the protection of materials or stored products, the amountof active components applied depends on the kind of application area andon the desired effect. Amounts customarily applied in the protection ofmaterials are 0.001 g to 2 kg, preferably 0.005 g to 1 kg, of activecomponents per cubic meter of treated material.

The user applies the composition according to the invention usually froma predosage device, a knapsack sprayer, a spray tank, a spray plane, oran irrigation system. Usually, the agrochemical composition is made upwith water, buffer, and/or further auxiliaries to the desiredapplication concentration and the ready-to-use spray liquor or theagrochemical composition according to the invention is thus obtained.Usually, 20 to 2000 liters, preferably 50 to 400 liters, of theready-to-use spray liquor are applied per hectare of agricultural usefularea.

In the mixtures and compositions, the compound ratios are advantageouslychosen so as to produce a synergistic effect.

The term “synergstic effect” is understood to refer in particular tothat defined by Colby's formula (Colby, S. R., “Calculating synergisticand antagonistic responses of herbicide combinations”, Weeds, 15, pp.20-22, 1967).

The term “synergistic effect” is also understood to refer to thatdefined by application of the Tammes method, (Tammes, P. M. L.,“Isoboles, a graphic representation of synergism in pesticides”,Nether!. J. Plant Pathol. 70, 1964).

According to the invention, the solid material (dry matter) of thebiopesticides (with the exception of oils such as Neem oil, Tagetes oil,etc.) are considered as active components (e.g. to be obtained afterdrying or evaporation of the extraction medium or filtration of thesuspension medium in case of liquid formulations of the microbialpesticides).

In accordance with the present invention, the weight ratios andpercentages used herein for a biological extract such as Quillay extractare based on the total weight of the dry content (solid material) of therespective extract(s).

The total weight ratios of compositions comprising at least onemicrobial pesticide in the form of viable microbial cells includingdormant forms, can be determined using the amount of CFU of therespective microorganism to calclulate the total weight of therespective active component with the following equation that 1×10¹⁰ CFUequals one gram of total weight of the respective active component.Colony forming unit is a measure of viable microbial cells, inparticular fungal and bacterial cells. In addition, here “CFU” may alsobe understood as the number of (juvenile) individual nematodes in caseof (entomopathogenic) nematode biopesticides, such as Steinernemafeltiae.

In the mixtures and compositions according to the invention the weightratio of the component 1) and the component 2) generally depends fromthe properties of the active components used, usually it is in the rangeof from 1:10,000 to 10,000:1, regularly in the range of from 1:100 to10,000:1, preferably in the range of from 1:100 to 5,000:1, morepreferably in the range of from 1:1 to 1,000:1, even more preferably inthe range of from 1:1 to 500:1 and in particular in the range of from10:1 to 300:1.

According to further embodiments of the mixtures and compositions, theweight ratio of the component 1) and the component 2) usually is in therange of from 20,000:1 to 1:10, often in the range of from 10,000:1 to1:1, regularly in the range of from 5,000:1 to 5:1, preferably in therange of from 5,000:1 to 10:1, more preferably in the range of from2,000:1 to 30:1, even more preferably in the range of from 2,000:1 to100:1 and in particular in the range of from 1,000:1 to 100:1.

According to further embodiments of the mixtures and compositions, theweight ratio of the component 1) and the component 2) usually is in therange of from 20,000:1 to 1:1,000, often in the range of from 10,000:1to 1:100, regularly in the range of from 5,000:1 to 1:1, preferably inthe range of from 5,000:1 to 10:1, more preferably in the range of from2,000:1 to 30:1, even more preferably in the range of from 2,000:1 to100:1 and in particular in the range of from 1,000:1 to 100:1.

According to further embodiments of the mixtures and compositions, theweight ratio of the component 1) and the component 2) usually is in therange of from 1:1 to 1:1000, often in the range of from 1:1 to 1:100,regularly in the range of from 1:1 to 1:50, preferably in the range offrom 1:1 to 1:20, more preferably in the range of from 1:1 to 1:10, evenmore preferably in the range of from 1:1 to 1:4 and in particular in therange of from 1:1 to 1:2.

In the abovementioned emodiments the total weight of component 1) iscalculated on the basis of of the amount of CFU of component 1), wherein1×10¹⁰ CFU equals one gram of total weight of component 1); and thetotal weight of component 2) in case of microorganisms is calculated onthe basis of of the amount of CFU of component 2), wherein 1×10¹⁰ CFUequals one gram of total weight of component 2).

These ratios are also suitable for inventive mixtures applied by seedtreatment.

The fungicidal action of the mixtures according to the invention can beshown by the tests described below.

A) Microtiter Plate Tests

The chemical pesticides II were formulated separately as a stocksolution having a concentration of 10000 ppm in dimethyl sulfoxide.

The stock solutions of the chemical pesticides II were mixed accordingto the ratio, diluted to the stated concentrations and pipetted onto afilter micro titer plate (MTP). A spore suspension of the pathogen (e.g.Botrytis cinerea, Septoria tritici, etc.) in e.g. aqueous biomaltsolution was added as well as different concentrations of spores orcells of microorganisms I and, if applicable, the microbial pesticidesII.

The measured parameters were compared to the growth of the activecompound-free control variant (100%) and the fungus-free and activecompound-free blank value to determine the relative growth in % of thepathogens in the respective active compounds.

The expected efficacies of active compound combinations were determinedusing Colby's formula (Colby, S. R., Calculating synergistic andantagonistic responses of herbicide combinations, Weeds, 15, pp. 20-22,1967) and compared with the observed efficacies.Colby's formula: E=x+y−x·y/100

-   E expected efficacy, expressed in % of the untreated control, when    using the mixture of the active components A and B at the    concentrations a and b-   x efficacy, expressed in % of the untreated control, when using the    active component A at the concentration a-   y efficacy, expressed in % of the untreated control, when using the    active component B at the concentration b.

We claim:
 1. A mixture comprising, as active components present in asynergistically effective amount: 1) at least one microorganism I of thegenus Bacillus that is B. amyloliquefaciens AP-188 (NRRL B-50331; NRRLB-50615); and 2) at least one pesticide II selected from pyraclostrobinand fluxapyroxad.
 2. The mixture of claim 1, wherein component 1) andcomponent 2) are present in a total weight ratio of from 10,000:1 to1:100; wherein the total weight of component 1) is calculated on thebasis of the amount of CFU of component 1), wherein 1×10¹⁰ CFU equalsone gram of total weight of component 1); and wherein the total weightof component 2) in case of microorganisms is calculated on the basis ofthe amount of CFU of component 2), wherein 1×10¹⁰ CFU of equals one gramof total weight of component 2).
 3. The mixture of claim 1, whereincomponent 2) is pyraclostrobin.
 4. The mixture of claim 3, whereincomponent 2) is fluxapyroxad.
 5. An agrochemical composition comprisingan auxiliary and the mixture of claim
 1. 6. A method for controllingphytopathogenic fungi, insects or other pests and/or improving thehealth of plants and/or regulating plant growth, comprising treating theplants, the plant propagation material or the soil with an effectiveamount of the mixture of claim
 1. 7. A method for controllingphytopathogenic fungi, insects or other pests and/or improving thehealth of plants and/or regulating plant growth, comprising treating theplants, the plant propagation material or the soil with an effectiveamount of the composition of claim
 5. 8. The method of claim 7, whereincomponent 2) is pyraclostrobin.
 9. The method of claim 7, whereincomponent 2) is pyraclostrobin and fluxapyroxad.
 10. A plant propagationmaterial, comprising the mixture of claim 1 in an amount of from 0.01 gto 10000 g per 100 kg of plant propagation material.
 11. A plantpropagation material treated with a composition comprising the mixtureof claim 1 in an amount of from 0.01 g to 10000 g of the mixture per 100kg of plant propagation material.
 12. A kit for preparing a usableagrochemical composition, the kit comprising as active components: 1) atleast one microorganism I of the genus Bacillus that is B.amyloliquefaciens AP-188 (NRRL B-50331; NRRL B-50615); and 2) at leastone pesticide II selected from.